Lubach D, Wawrzyniak-Schulz A, Neukam D, Nissen S
Department of Dermatology (Hautklinik Linden), Medizinische Hochschule, Hannover, F.R.G.
Br J Dermatol. 1990 Aug;123(2):179-85. doi: 10.1111/j.1365-2133.1990.tb01845.x.
The initial lymph vessels, the lymph capillaries and precollectors, are difficult to detect in normal skin using routine histological methods. A method is described in which specimens of the skin are fixed in an expanded condition. In 1.05 microns thick plastic-embedded sections the dilated lymph vessels of the skin can be demonstrated. The dermal lymph vessel network can be seen following the subepidermal injection of a coloured solution. The endothelial cells and subendothelial fibrous network can be examined using electron microscopy.
最初的淋巴管，即毛细淋巴管和集合前淋巴管，使用常规组织学方法在正常皮肤中难以检测到。本文描述了一种将皮肤标本在扩张状态下固定的方法。在1.05微米厚的塑料包埋切片中，可以显示皮肤扩张的淋巴管。在表皮下注射有色溶液后，可以看到真皮淋巴管网络。可以使用电子显微镜检查内皮细胞和内皮下纤维网络。
