• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

hERG 钾通道 S4-S5 连接环的溶液结构。

The solution structure of the S4-S5 linker of the hERG potassium channel.

机构信息

Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), Singapore 138669, Singapore.

出版信息

J Pept Sci. 2012 Feb;18(2):140-5. doi: 10.1002/psc.1427. Epub 2011 Nov 3.

DOI:10.1002/psc.1427
PMID:22052838
Abstract

The human ether-à-go-go related gene (hERG) encodes a protein that forms a voltage-gated potassium channel and plays an important role in the heart by controlling the rapid delayed rectifier K(+) current (I(Kr)). The S4-S5 linker was shown to be important for the gating of the hERG channel. Nuclear magnetic resonance study showed that a peptide derived from the S4-S5 linker had no well-ordered structure in aqueous solution and adopted a 3(10) -helix (E544-Y545-G546) structure in detergent micelles. The existence of an amphipathic helix was confirmed, which may be important for interaction with cell membrane. Close contact between side chains of residues R541 and E544 was observed, which may be important for its regulation of channel gating.

摘要

人类 ether-à-go-go 相关基因 (hERG) 编码一种形成电压门控钾通道的蛋白质，通过控制快速延迟整流钾电流 (I(Kr)) 在心脏中发挥重要作用。S4-S5 连接子被证明对 hERG 通道的门控很重要。核磁共振研究表明，源自 S4-S5 连接子的肽在水溶液中没有有序结构，并且在去污剂胶束中采用 3(10)-螺旋 (E544-Y545-G546) 结构。确认存在一个两亲螺旋，这可能对与细胞膜的相互作用很重要。观察到残基 R541 和 E544 的侧链之间的紧密接触，这可能对其调节通道门控很重要。

相似文献

1
The solution structure of the S4-S5 linker of the hERG potassium channel.hERG 钾通道 S4-S5 连接环的溶液结构。
J Pept Sci. 2012 Feb;18(2):140-5. doi: 10.1002/psc.1427. Epub 2011 Nov 3.
2
Structure of the HERG K+ channel S5P extracellular linker: role of an amphipathic alpha-helix in C-type inactivation.HERG钾离子通道S5P胞外连接子的结构：两亲性α螺旋在C型失活中的作用
J Biol Chem. 2003 Oct 24;278(43):42136-48. doi: 10.1074/jbc.M212824200. Epub 2003 Aug 5.
3
Potential role of the membrane in hERG channel functioning and drug-induced long QT syndrome.膜在人乙醚-a- go-相关基因（hERG）通道功能及药物诱导的长QT综合征中的潜在作用。
Biochim Biophys Acta. 2010 Sep;1798(9):1651-62. doi: 10.1016/j.bbamem.2010.05.019. Epub 2010 May 25.
4
Structural insight into the transmembrane segments 3 and 4 of the hERG potassium channel.人乙醚相关基因（hERG）钾通道跨膜片段3和4的结构解析
J Pept Sci. 2014 Dec;20(12):935-44. doi: 10.1002/psc.2704. Epub 2014 Oct 20.
5
Structural analysis of the S4-S5 linker of the human KCNQ1 potassium channel.人类KCNQ1钾通道S4-S5连接区的结构分析
Biochem Biophys Res Commun. 2015 Jan 2;456(1):410-4. doi: 10.1016/j.bbrc.2014.11.097. Epub 2014 Dec 2.
6
NMR solution structure of the N-terminal domain of hERG and its interaction with the S4-S5 linker.hERG 氨基末端结构域的 NMR 溶液结构及其与 S4-S5 连接子的相互作用。
Biochem Biophys Res Commun. 2010 Dec 3;403(1):126-32. doi: 10.1016/j.bbrc.2010.10.132. Epub 2010 Nov 3.
7
Solution structures of the cytoplasmic linkers between segments S4 and S5 (S4-S5) in domains III and IV of human brain sodium channels in SDS micelles.人脑中钠通道结构域III和IV中S4和S5片段（S4-S5）之间胞质连接体在SDS胶束中的溶液结构。
J Pept Res. 2001 Sep;58(3):193-203. doi: 10.1034/j.1399-3011.2001.00912.x.
8
Purification and structural characterization of the voltage-sensor domain of the hERG potassium channel.人乙醚 - 去极化相关基因（hERG）钾通道电压传感器结构域的纯化与结构表征
Protein Expr Purif. 2012 Dec;86(2):98-104. doi: 10.1016/j.pep.2012.09.003. Epub 2012 Oct 3.
9
The S4-S5 linker acts as a signal integrator for HERG K+ channel activation and deactivation gating.S4-S5 连接子充当 HERG K+ 通道激活和失活门控的信号整合器。
PLoS One. 2012;7(2):e31640. doi: 10.1371/journal.pone.0031640. Epub 2012 Feb 16.
10
Three-dimensional structure of the S4-S5 segment of the Shaker potassium channel.果蝇钾通道S4-S5片段的三维结构。
Biophys J. 2002 Jun;82(6):2995-3002. doi: 10.1016/S0006-3495(02)75640-3.

引用本文的文献

1
Structure of the Cyclic Nucleotide-Binding Homology Domain of the hERG Channel and Its Insight into Type 2 Long QT Syndrome.人乙醚 - 去极化相关基因（hERG）通道环核苷酸结合同源结构域的结构及其对2型长QT综合征的启示
Sci Rep. 2016 Mar 30;6:23712. doi: 10.1038/srep23712.
2
Conservation analysis of residues in the S4-S5 linker and the terminal part of the S5-P-S6 pore modulus in Kv and HCN channels: flexible determinants for the electromechanical coupling.Kv和HCN通道中S4-S5连接区及S5-P-S6孔模末端部分残基的保守性分析：机电耦合的灵活决定因素
Pflugers Arch. 2015 Oct;467(10):2069-79. doi: 10.1007/s00424-014-1647-3. Epub 2014 Nov 15.
3
Structure of the C-terminal region of the Frizzled receptor 1 in detergent micelles.
卷曲受体 1 胞内 C 端区域在去污剂胶束中的结构。
Molecules. 2013 Jul 22;18(7):8579-90. doi: 10.3390/molecules18078579.
4
Insight into the modulation of Shaw2 Kv channels by general anesthetics: structural and functional studies of S4-S5 linker and S6 C-terminal peptides in micelles by NMR.深入了解全身麻醉药对Shaw2钾离子通道的调节作用：通过核磁共振对胶束中S4-S5连接肽和S6 C末端肽进行结构和功能研究。
Biochim Biophys Acta. 2013 Feb;1828(2):595-601. doi: 10.1016/j.bbamem.2012.09.025. Epub 2012 Sep 29.
5
Voltage-dependent gating of HERG potassium channels.HERG钾通道的电压依赖性门控
Front Pharmacol. 2012 May 8;3:83. doi: 10.3389/fphar.2012.00083. eCollection 2012.
6
Insights into hERG K+ channel structure and function from NMR studies.NMR 研究揭示 hERG K+ 通道结构与功能。
Eur Biophys J. 2013 Jan;42(1):71-9. doi: 10.1007/s00249-012-0808-6. Epub 2012 May 3.
7
The S4-S5 linker acts as a signal integrator for HERG K+ channel activation and deactivation gating.S4-S5 连接子充当 HERG K+ 通道激活和失活门控的信号整合器。
PLoS One. 2012;7(2):e31640. doi: 10.1371/journal.pone.0031640. Epub 2012 Feb 16.