Yan Li, Xiao Lei, Wei Jun-Feng, Sun Jian, Wang Zhan-Hui, Hou Jin-Lin
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Gan Zang Bing Za Zhi. 2011 Jun;19(6):423-6.
To investigate the relationship between the mutation patterns of rtM204V/I (methionine to valine or isoleucine at position rt204 of reverse transcriptase domain) in hepatitis B virus (HBV) polymerase gene and HBV genotypes.
A total of 2849 HBV complete genome sequences were retrieved from the GenBank/EMBL/DDBJ. HBV genotypes were determined by using MEGA4 software. The amino acid sequences of the reverse transcriptase (RT) domain were aligned. Data were analyzed using SPSS 13.0. RESULTS Among the 2849 HBV complete genome sequences, 217 strains with Y (I/V) DD were identified. Of them, 120 had YIDD mutation and the genotype/subgenotype distribution was as follows: A (2), B(B2 19), C(C1 1, C2 78, C5 1), D(17), E(1), G(1); 97 had YVDD mutation and the genotype/subgenotype distribution was as follows: A(17), B(B2 22), C(C1 3, C2 48), D(3), G(3), H(1). There is a significant difference in the mutation patterns of Y (I/V) DD among genotypes of A-D, A-C, and between genotype A and B, P < 0.01.There is a difference in the mutation pattern of Y (I/V) DD among genotypes of B-D, between genotype C and D, P < 0.05. Genotype A has a higher tendency to develop YVDD mutation, whereas genotype D has a higher frequency to develop YIDD mutation. The rtM204V-rtL180M mutations were more frequently found in subgenotype B2 than in subgenotype C2 while the rtM204V-rtL180M-rtV173L mutations were more associated with subgenotype C2 (P < 0.01).
Different HBV genotype/subgenotype may select different mutation pattern in the YMDD domain. Subgenotype C2 is more diversity and complexity than other HBV genotypes/subgenotypes.
探讨乙型肝炎病毒（HBV）聚合酶基因中逆转录酶结构域rt204位点（甲硫氨酸突变为缬氨酸或异亮氨酸）的突变模式与HBV基因型之间的关系。
从GenBank/EMBL/DDBJ中检索到2849条HBV全基因组序列。使用MEGA4软件确定HBV基因型。对逆转录酶（RT）结构域的氨基酸序列进行比对。采用SPSS 13.0软件进行数据分析。结果：在2849条HBV全基因组序列中，鉴定出217株Y（I/V）DD突变株。其中，120株发生YIDD突变，其基因型/亚基因型分布如下：A（2株）、B（B2 19株）、C（C1 1株、C2 78株、C5 1株）、D（17株）、E（1株）、G（1株）；97株发生YVDD突变，其基因型/亚基因型分布如下：A（17株）、B（B2 22株）、C（C1 3株、C2 48株）、D（3株）、G（3株）、H（1株）。A - D、A - C基因型以及A和B基因型之间Y（I/V）DD的突变模式存在显著差异，P < 0.01。B - D基因型之间以及C和D基因型之间Y（I/V）DD的突变模式存在差异，P < 0.05。基因型A发生YVDD突变的倾向较高，而基因型D发生YIDD突变的频率较高。rtM204V - rtL180M突变在B2亚基因型中比在C2亚基因型中更常见，而rtM204V - rtL180M - rtV173L突变与C2亚基因型更相关（P < 0.01）。
不同的HBV基因型/亚基因型可能在YMDD结构域选择不同的突变模式。C2亚基因型比其他HBV基因型/亚基因型具有更多样性和复杂性。
