Kimura E, Mattei D, di Santi S M, Scherf A
Departamento de Parasitologia, ICBII, Universidade de Sâo Paulo, Cidade Universitaria-Cep, Brasil.
Gene. 1990 Jul 2;91(1):57-62. doi: 10.1016/0378-1119(90)90162-k.
We studied the diversity of the polymorphic 195-kDa antigen (p190) of Plasmodium from infected individuals. Genomic parasite DNA was extracted from the blood of 30 donors from different endemic areas of Brazil. The 5' region, encoding the polymorphic N-terminal part of p190 was analysed following polymerase chain reaction (PCR). Multiple infections of genetically distinct parasites could be detected within infected malaria patients. Sequence analysis and oligodeoxyribonucleotide typing of the PCR products demonstrated the prevalence of a third polymorphic form of p190.
我们研究了来自受感染个体的疟原虫多态性195-kDa抗原（p190）的多样性。从巴西不同流行地区的30名献血者的血液中提取了基因组寄生虫DNA。通过聚合酶链反应（PCR）分析了编码p190多态性N端部分的5'区域。在受感染的疟疾患者中可以检测到遗传上不同的寄生虫的多重感染。PCR产物的序列分析和寡脱氧核糖核苷酸分型表明p190的第三种多态形式普遍存在。
