Nair I, Mahadevan P R
Foundation for Medical Research, Worli, Bombay, India.
Int J Lepr Other Mycobact Dis. 1990 Sep;58(3):540-7.
Peritoneal macrophages from randombred, Swiss white mice, when cultured and infected with Mycobacterium leprae for 24 hours, are able to show the presence of antigen(s) with binding affinity to antibodies present in the sera of bacteriologically positive, lepromatous leprosy patients. Such antibodies are not seen in sera from normal and healthy persons, tuberculoid leprosy patients, or long-term-treated, bacteriologically negative, lepromatous leprosy patients. The production of the antigen(s) is blocked by the anti-M leprae drug rifampin. Other mycobacteria when incubated with macrophages from mice show very little antigens in the lysate but the antigens have an equal affinity for antibodies in sera from both normal individuals and lepromatous patients. Only the lysates from macrophages exposed to live M. leprae could discriminate and could exhibit differential binding to sera from leprosy patients compared to sera from normal individuals. This antigen(s) does not have any binding ability to the monoclonal antibodies available to the antigens of M. leprae identified at present and shown to be specific to M. leprae. This indicates a separate identity of this product which has potential for further exploitation in exploring host-pathogen interactions related specifically to the leprosy infection and the tolerance of M. leprae inside cells.
将随机繁殖的瑞士小白鼠的腹腔巨噬细胞进行培养，并感染麻风分枝杆菌24小时后，这些巨噬细胞能够显示出与细菌学检测呈阳性的瘤型麻风患者血清中存在的抗体具有结合亲和力的抗原。在正常健康人、结核样型麻风患者或长期治疗后细菌学检测呈阴性的瘤型麻风患者的血清中未发现此类抗体。抗麻风药物利福平可阻断该抗原的产生。其他分枝杆菌与小鼠巨噬细胞一起孵育时，裂解物中显示的抗原极少，且这些抗原对正常人和瘤型麻风患者血清中的抗体具有同等亲和力。只有暴露于活麻风分枝杆菌的巨噬细胞裂解物能够区分，并与麻风患者血清相比，对正常个体血清表现出不同的结合。这种抗原与目前可获得的针对已鉴定的麻风分枝杆菌抗原的单克隆抗体没有任何结合能力，且这些单克隆抗体已证明对麻风分枝杆菌具有特异性。这表明该产物具有独特的特性，在探索与麻风感染及麻风分枝杆菌在细胞内的耐受性相关的宿主 - 病原体相互作用方面具有进一步开发的潜力。
