Course of Biological Science and Technology, The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
Plant Signal Behav. 2011 Nov;6(11):1824-7. doi: 10.4161/psb.6.11.17478. Epub 2011 Nov 1.
The phytohormone brassinosteroid (BR) is crucial for plant growth and development. Although genetic and molecular approaches have improved understanding of the cellular BR signaling pathway, we still do not have sufficient knowledge about the function of BR. Therefore, proteomic analysis was used to elucidate BR signaling and gene expression in the nuclei of suspended Arabidopsis cells treated with brassinolide, a bioactive BR, or brassinazole, a BR biosynthesis inhibitor. Interestingly, chromatin remodeling-related proteins, the abundance of which was altered in response to cellular BR levels, were identified. This suggested that BR-induced gene expression is regulated not only by transcription factors directly binding to cis-elements, but also by chromatin remodeling in response to BR signaling. In this addendum, we summarize the functions of our identified nuclear proteins in chromatin remodeling and discuss the need for chromatin remodeling regulated by BR signal transduction for expression of BR-induced genes.
植物激素油菜素内酯（BR）对植物的生长和发育至关重要。尽管遗传和分子方法已经提高了对细胞 BR 信号通路的理解，但我们仍然没有足够的关于 BR 功能的知识。因此，蛋白质组学分析被用来阐明悬浮拟南芥细胞细胞核中 BR 信号和基因表达，这些细胞用油菜素内酯（一种生物活性 BR）或油菜素唑（一种 BR 生物合成抑制剂）处理。有趣的是，鉴定到了与染色质重塑相关的蛋白质，其丰度响应细胞 BR 水平而改变。这表明 BR 诱导的基因表达不仅受到直接与顺式元件结合的转录因子的调控，还受到染色质重塑的调控，以响应 BR 信号。在这个增刊中，我们总结了我们鉴定的核蛋白在染色质重塑中的功能，并讨论了 BR 信号转导调控染色质重塑对于表达 BR 诱导基因的必要性。
