通过微阵列研究耐药酶对氨基糖苷类抗生素的修饰作用。
Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray.
作者信息
Disney Matthew D
机构信息
Department of Chemistry, The Scripps Research Institute, Scripps Florida, Jupiter, FL, USA.
出版信息
Methods Mol Biol. 2012;808:303-20. doi: 10.1007/978-1-61779-373-8_21.
Abstract
Widespread bacterial resistance to antibiotics is a significant public health concern. To remain a step ahead of evolving bacteria, new methods to study resistance to antibacterials and to uncover novel antibiotics that evade resistance are urgently needed. Herein, microarray-based methods that have been developed to study aminoglycoside modification by resistance-causing enzymes are reviewed. These arrays can also be used to study the binding of aminoglycoside antibiotics to a mimic of their therapeutic target, the rRNA aminoacyl site (A-site), and how modification by resistance-causing enzymes affects their abilities to bind RNA.
摘要
细菌对抗生素的广泛耐药性是一个重大的公共卫生问题。为了在不断进化的细菌面前保持领先，迫切需要研究抗菌药物耐药性的新方法以及发现能规避耐药性的新型抗生素。本文综述了基于微阵列的方法，这些方法已被开发用于研究耐药酶对氨基糖苷类药物的修饰作用。这些阵列还可用于研究氨基糖苷类抗生素与其治疗靶点——核糖体RNA氨酰基位点（A位点）的模拟物的结合情况，以及耐药酶的修饰作用如何影响它们与RNA的结合能力。
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