苜蓿银纹夜蛾核型多角体病毒基因组在昆虫细胞中的表达。

The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells.

作者信息

Li J A, Happ B, Schetter C, Oellig C, Hauser C, Kuroda K, Knebel-Mörsdorf D, Klenk H D, Doerfler W

机构信息

Institute of Genetics, University of Cologne, F.R.G.

出版信息

Vet Microbiol. 1990 Jun;23(1-4):73-8. doi: 10.1016/0378-1135(90)90137-k.

DOI:10.1016/0378-1135(90)90137-k

PMID:2205973

Abstract

This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. (1) On the mode of transcription of the AcNPV genome in insect cells. (2) Translation of proteins encoded in the 81.2 to 85.0 map unit segment of AcNPV. (3) Inserts of insect cell DNA in the AcNPV genome. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.

摘要

本报告概述了我们实验室最近发表的关于昆虫杆状病毒苜蓿银纹夜蛾核型多角体病毒（AcNPV）分子生物学的研究工作。以下研究内容已被总结：（1）AcNPV基因组在昆虫细胞中的转录模式。（2）AcNPV基因组81.2至85.0图谱单位区段所编码蛋白质的翻译。（3）昆虫细胞DNA插入AcNPV基因组的情况。（4）在草地贪夜蛾昆虫细胞中表达流感（禽瘟）病毒血凝素，并成功免疫鸡。（5）通过重组AcNPV在昆虫幼虫中合成流感病毒血凝素。这种昆虫病毒系统将继续作为昆虫分子生物学研究的模型。此外，杆状病毒系统已被公认为一种非常高效且安全的真核表达载体。