Wöhrl B M, Sprenger G A, Lengeler J W
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Arch Microbiol. 1990;154(2):162-7. doi: 10.1007/BF00423327.
Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking D-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an L-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from D-fructose via D-fructose-1-phosphate and D-mannitol-1-phosphate to D-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively.
从缺乏D-果糖-1-磷酸激酶（E.C. 2.7.1.3.）的大肠杆菌K12的fruK（以前称为fpk）突变体开始，在引入来自肺炎克雷伯菌的编码L-山梨糖-1-磷酸还原酶的克隆基因sorE后，分离出果糖阳性衍生物。新途径显示从D-果糖经D-果糖-1-磷酸和D-甘露醇-1-磷酸到D-果糖6-磷酸。它分别涉及大肠杆菌K12的fru和mtl操纵子以及肺炎克雷伯菌的sor操纵子中编码的转运系统和酶。
