Department of Health Care, Japanese Red Cross Medical Center, 4-1-22 Hiroo, Shibuya-ku, Tokyo 150-8935, Japan.
Clin Biochem. 2012 Jan;45(1-2):72-6. doi: 10.1016/j.clinbiochem.2011.10.013. Epub 2011 Oct 28.
High-molecular-weight (HMW)-adiponectin is an active and powerful predictor of atherosclerosis. Unfortunately, there is currently no reliable epidemiological reference level data available in Japan. The current ELISA method for HMW-adiponectin measurement is tedious as it requires several sample pre-treatment steps. We studied the reference level of HMW-adiponectin measured by a simple, fully-automated CLEIA.
The study subjects were 858 men and 793 women who underwent health check-ups at a Japanese Red Cross Medical Center. We investigated the relation between HMW-adiponectin and metabolic parameters, including carotid ultrasonography.
The mean values of HMW-adiponectin were 3.11 ± 1.99 μg/ml in men, and 5.52 ± 3.07 μg/ml in women. There were significant differences (p<0.001) in relation to gender and age, where levels gradually increased with age. HMW-adiponectin was 3.18 ± 1.45 μg/ml in male subjects without carotid lesions, and 2.28 ± 0.90 μg/ml in male subjects with lesions.
HMW-adiponectin measured by simple CLEIA could be useful for routine risk assessment.
高分子量（HMW）-脂联素是动脉粥样硬化的一个活跃且强有力的预测因子。不幸的是，目前在日本还没有可靠的流行病学参考水平数据。目前用于 HMW-脂联素测量的 ELISA 方法繁琐，因为它需要几个样品预处理步骤。我们研究了通过简单的全自动 CLEIA 测量 HMW-脂联素的参考水平。
研究对象为在日本红十字医疗中心接受健康检查的 858 名男性和 793 名女性。我们调查了 HMW-脂联素与代谢参数之间的关系，包括颈动脉超声检查。
男性的 HMW-脂联素平均值为 3.11 ± 1.99 μg/ml，女性为 5.52 ± 3.07 μg/ml。HMW-脂联素与性别和年龄存在显著差异（p<0.001），随着年龄的增长，水平逐渐升高。无颈动脉病变的男性 HMW-脂联素为 3.18 ± 1.45 μg/ml，有病变的男性 HMW-脂联素为 2.28 ± 0.90 μg/ml。
通过简单 CLEIA 测量的 HMW-脂联素可用于常规风险评估。
