Lametsch R, Knudsen J C, Ertbjerg P, Oksbjerg N, Therkildsen M
Department of Food Science, Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
Meat Sci. 2007 Apr;75(4):719-24. doi: 10.1016/j.meatsci.2006.10.002. Epub 2006 Nov 22.
Multi angle light scattering was used to determine the myofibril fragmentation of pig longissimus dorsi muscle which was then compared with results from the common turbidity method. The method is based on measurement of the myofibril particle size distribution with the use of a special optical unit containing several individual detectors. The method was able to determine post-mortem changes in a pig muscle homogenate without purification of the myofibrils and is therefore simpler and much faster than the traditional turbidity method. There was a significant correlation (p<0.01) between Warner-Bratzler shear force (WBSF) and particle size distribution. The root mean square error of prediction was found to be 6.1N (10-15% of the measured WBSF) when multivariate data analysis was used to make a prediction model for WBSF. Multi angle light scattering is very useful for estimation of myofibril fragmentation since the method is fast and the sample preparation is simple.
采用多角度光散射法测定猪背最长肌的肌原纤维破碎情况，然后将结果与传统比浊法的结果进行比较。该方法基于使用包含多个独立探测器的特殊光学单元测量肌原纤维粒度分布。该方法能够在不纯化肌原纤维的情况下测定猪肌肉匀浆的宰后变化，因此比传统比浊法更简单、更快。沃纳-布拉茨勒剪切力（WBSF）与粒度分布之间存在显著相关性（p<0.01）。当使用多变量数据分析建立WBSF预测模型时，预测的均方根误差为6.1N（占测量WBSF的10-15%）。多角度光散射法对于肌原纤维破碎的评估非常有用，因为该方法快速且样品制备简单。
