Bendixen Emøke
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, P.O. Box 50, 8830 Tjele, Denmark.
Meat Sci. 2005 Sep;71(1):138-49. doi: 10.1016/j.meatsci.2005.03.013.
Characterising the function of genes is a major challenge in the post-genomic era. Post-genomic tools and technologies have dramatically changed the experimental approaches by which complex biological systems can be characterised. Proteomics is an important cornerstone in functional genome characterisation, and like all other functional genomics tools, including transcriptomics and metabolomics, the aim of proteome studies is to translate genome information into useful biological insight, that will allow scientists to build and test better hypotheses, with the ultimate goal to find better solutions to challenges in food production, medicine and environmental management. In agricultural sciences as well as in all other life sciences, the implementation of proteomics and the other post-genomic tools is an important step towards achieving better product quality and a more sustainable animal production. The aim of this review is to introduce the developing field of proteomics, and to discuss the use of proteomics in meat science projects. The most frequently used technologies for characterising cellular protein expression patterns will be introduced, and some early examples of applying proteomics to meat quality research will be discussed.
在基因组时代之后，确定基因的功能是一项重大挑战。基因组时代之后的工具和技术极大地改变了用于表征复杂生物系统的实验方法。蛋白质组学是功能基因组表征的重要基石，与所有其他功能基因组学工具（包括转录组学和代谢组学）一样，蛋白质组研究的目的是将基因组信息转化为有用的生物学见解，使科学家能够构建和检验更好的假设，最终目标是找到应对食品生产、医学和环境管理挑战的更好解决方案。在农业科学以及所有其他生命科学中，实施蛋白质组学和其他基因组时代之后的工具是实现更好产品质量和更可持续动物生产的重要一步。本综述的目的是介绍蛋白质组学这一发展中的领域，并讨论蛋白质组学在肉类科学项目中的应用。将介绍用于表征细胞蛋白质表达模式的最常用技术，并讨论一些将蛋白质组学应用于肉质研究的早期实例。
