Young O A, West J, Hart A L, van Otterdijk F F H
Department of Applied Science, Auckland University of Technology, Auckland, New Zealand.
Meat Sci. 2004 Feb;66(2):493-8. doi: 10.1016/S0309-1740(03)00140-2.
A patented method of rapidly determining the ultimate pH from approximate glycolytic potential of muscles of slaughtered animals has been devised. The method is based on the rapid hydrolysis of muscle glycogen to glucose by the enzyme amyloglucosidase and subsequent measurement of the liberated glucose. In acetate buffer at pH 4.5, glucose concentration can be determined in 30 s with domestic meters for diabetes control. The meter response differed from that of glucose in blood, but was linear with concentration. In slurries comprising homogenised meat in acetate buffer and added glucose, a similar linear response was obtained. Amyloglucosidase was capable of rapidly hydrolysing glycogen to glucose in such slurries. In the 24 h following slaughter, a decrease in glycogen, as determined by glucose, occurred in parallel with the decline in pH. At the same time, lactate progressively accumulated as expected. Values for the approximate glycolytic potential and (by calibration) ultimate pH, were obtained on prerigor muscle within 7 min of muscle sampling in an industrial environment. The method is suitable for on-line application in beef abattoirs particularly those employing hot boning where ultimate must be known at the grading point.
已设计出一种专利方法，可根据屠宰动物肌肉的近似糖酵解潜力快速测定最终pH值。该方法基于淀粉葡萄糖苷酶将肌肉糖原快速水解为葡萄糖，随后测量释放出的葡萄糖。在pH 4.5的醋酸盐缓冲液中，使用家用糖尿病控制血糖仪可在30秒内测定葡萄糖浓度。血糖仪的响应与血液中葡萄糖的响应不同，但与浓度呈线性关系。在含有醋酸盐缓冲液中匀浆肉并添加葡萄糖的浆液中，也获得了类似的线性响应。淀粉葡萄糖苷酶能够在这种浆液中迅速将糖原水解为葡萄糖。在屠宰后的24小时内，通过葡萄糖测定的糖原减少与pH值下降同时发生。与此同时，乳酸按预期逐渐积累。在工业环境中，在肌肉采样后7分钟内，即可在尸僵前肌肉上获得近似糖酵解潜力和（通过校准）最终pH值。该方法适用于牛肉屠宰场的在线应用，特别是那些采用热剔骨的屠宰场，在分级点必须知道最终pH值。
