Cho K J, Myong N H, Jang J J
Department of Anatomical Pathology, Korea Cancer Center Hospital.
J Korean Med Sci. 1990 Mar;5(1):13-7. doi: 10.3346/jkms.1990.5.1.13.
The effects of ethyl alcohol and pig serum administration on the development of preneoplastic hepatic enzyme-altered foci were examined in an in vivo mid-term assay system. Rats were initially given a single dose (200 mg/Kg) intraperitoneal injection of diethylnitrosamine (DEN). Two weeks later, treatment was started with 10% ethanol + 10% sucrose solution, 10% sucrose solution, or tap water as drinking water for 6 weeks with or without intraperitoneal injection of porcine serum twice a week. All rats were subjected to a two-thirds partial hepatectomy at week 3. The modification potentials were evaluated by comparing the number and area per cm2 of glutathione S-transferase placental form-positive (GST-P+) foci in the liver of each group. As a result, ethanol significantly enhanced the development of GST-P+ foci. Unfortunately, the porcine serum injection produced no hepatic fibrosis and no significant alteration in GST-P+ foci.
在一个体内中期检测系统中，研究了给予乙醇和猪血清对癌前肝酶改变灶发展的影响。大鼠最初腹腔注射一次剂量（200mg/Kg）的二乙基亚硝胺（DEN）。两周后，开始用10%乙醇+10%蔗糖溶液、10%蔗糖溶液或自来水作为饮用水处理6周，同时每周腹腔注射猪血清两次，或不注射。所有大鼠在第3周接受三分之二部分肝切除术。通过比较每组肝脏中谷胱甘肽S-转移酶胎盘型阳性（GST-P+）灶的数量和每平方厘米面积来评估其改变潜能。结果显示，乙醇显著增强了GST-P+灶的发展。遗憾的是，注射猪血清未产生肝纤维化，且GST-P+灶无显著改变。
