Seböková E, Kolena J
Physiol Bohemoslov. 1979;28(1):25-30.
Stimulation of cAMP synthesis by prostaglandins E series in the rat ovary is consistent with the presence of a prostaglandin receptor in this tissue. Prostaglandin binding sites with specificity for PGE1 in vitro incubation systems have been demonstrated in rat ovary slices and corpora lutea. The binding of 3H-PGE1 was progressively inhibited with increasing amounts of unlabelled PGE1 and PGE2. PGF2alpha inhibitory effect was markedly smaller than that of PGE. 3H-PGE1 binding to the ovary was higher in 3-day-old rats than in 5-day-old and adult animals, when the highest binding was present in estrus. The specific binding of 3H-PGE1 to rat corpora lutea (CL) decreased on days 11 and 13 of pregnancy and then gradually returned to the level found on day 1 during the second half of gestation. This binding of labelled prostaglandin during pregnancy has been studied in relation to the PGE1 stimulation of cAMP synthesis in rat corpora lutea, but no consistent changes were observed in responsiveness.
前列腺素E系列对大鼠卵巢中环磷酸腺苷（cAMP）合成的刺激作用与该组织中存在前列腺素受体相一致。在大鼠卵巢切片和黄体的体外孵育系统中，已证实存在对前列腺素E1具有特异性的前列腺素结合位点。随着未标记的前列腺素E1和前列腺素E2量的增加，3H-前列腺素E1的结合逐渐受到抑制。前列腺素F2α的抑制作用明显小于前列腺素E。3日龄大鼠卵巢对3H-前列腺素E1的结合高于5日龄和成年动物，成年动物在发情期结合最高。在妊娠第11天和第13天，3H-前列腺素E1与大鼠黄体（CL）的特异性结合下降，然后在妊娠后半期逐渐恢复到第1天的水平。已研究了妊娠期间标记前列腺素的这种结合与前列腺素E1对大鼠黄体中cAMP合成的刺激作用之间的关系，但未观察到反应性的一致变化。
