The Sobell Department of Motor Neuroscience and Movement Disorders, UCL Institute of Neurology, London, UK.
J Cell Biochem. 2012 Mar;113(3):1044-53. doi: 10.1002/jcb.23437.
Increased recent research activity in exercise physiology has dramatically improved our understanding of skeletal muscle development and physiology in both health and disease. Advances in bioengineering have enabled the development of biomimetic 3D in vitro models of skeletal muscle which have the potential to further advance our understanding of the fundamental processes that underpin muscle physiology. As the principle structural protein of the extracellular matrix, collagen-based matrices are popular tools for the creation of such 3D models but the custom nature of many reported systems has precluded their more widespread adoption. Here we present a simple, reproducible iteration of an established 3D in vitro model of skeletal muscle, demonstrating both the high levels of reproducibility possible in this system and the improved cellular architecture of such constructs over standard 2D cell culture techniques. We have used primary rat muscle cells to validate this simple model and generate comparable data to conventional established cell culture techniques. We have optimized culture parameters for these cells which should provide a template in this 3D system for using muscle cells derived from other donor species and cell lines.
最近，运动生理学领域的研究活动不断增加，极大地提高了我们对健康和疾病状态下骨骼肌发育和生理学的理解。生物工程学的进步使得仿生三维体外骨骼肌模型得以发展，这有可能进一步促进我们对支撑肌肉生理学的基本过程的理解。胶原蛋白基质作为细胞外基质的主要结构蛋白，是创建此类 3D 模型的常用工具，但许多报道的系统的定制性质限制了它们的更广泛应用。在这里，我们展示了一种简单、可重复的骨骼肌三维体外模型的迭代，该模型证明了该系统中可能具有的高度可重复性，以及与标准二维细胞培养技术相比，此类构建体的细胞结构得到了改善。我们使用原代大鼠肌肉细胞对这个简单的模型进行了验证，并生成了与传统的细胞培养技术相当的数据。我们已经对这些细胞的培养参数进行了优化，这应该为使用源自其他供体物种和细胞系的肌肉细胞在这个三维系统中提供了一个模板。
