Programa de Neurociências, Departamento de Neurobiologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil.
Neuroscience. 2012 Jan 3;200:223-36. doi: 10.1016/j.neuroscience.2011.10.034. Epub 2011 Oct 25.
Interleukin-2 (IL-2) plays regulatory functions both in immune and nervous system. However, in the visual system, little is known about the cellular types which respond to IL-2 and its effects. Herein, we investigated the influence of IL-2 in the development of central visual pathways. Lister Hooded rats were submitted to multiple (at postnatal days [PND]7/10/13) or single (at PND10) intravitreous injections of phosphate-buffered saline (PBS) (vehicle), zymosan, or IL-2. IL-2 receptor α subunit was detected in the whole postnatal retina. Chronic treatment with either PBS or IL-2 increases retinal glial fibrillary acidic protein (GFAP) expression, induces intravitreous inflammation revealed by the presence of macrophages, and results in a slight rearrangement of retinotectal axons. Acute zymosan treatment disrupts retinotectal axons distribution, confirming the influence of inflammation on retinotectal pathway reordering. Furthermore, acute IL-2 treatment increases GFAP expression in the retina without inflammation and produces a robust sprouting of the intact uncrossed retinotectal pathway. No difference was observed in glial cells activity in superior colliculus. Taken together, these data suggest that inflammation and interleukin-2 modulate retinal ganglion cells development and the distribution of their axons within central targets.
白细胞介素-2 (IL-2) 在免疫系统和神经系统中都具有调节功能。然而，在视觉系统中，对于哪些细胞类型对 IL-2 及其作用作出反应，人们知之甚少。在此，我们研究了 IL-2 对中枢视觉通路发育的影响。利斯特 Hooded 大鼠在出生后第 7/10/13 天（PND）接受多次（PND10）或单次（PND10）玻璃体内注射磷酸盐缓冲盐水（PBS）（载体）、酵母聚糖或 IL-2。在整个新生视网膜中检测到 IL-2 受体 α 亚基。用 PBS 或 IL-2 进行慢性处理会增加视网膜神经胶质纤维酸性蛋白 (GFAP) 的表达，引起玻璃体内炎症（巨噬细胞的存在），并导致视束轴突的轻微重排。急性酵母聚糖处理破坏了视束轴突的分布，证实了炎症对视束通路重排的影响。此外，急性 IL-2 处理会增加无炎症的视网膜中 GFAP 的表达，并导致完整未交叉的视束通路的大量发芽。在外侧丘脑中，神经胶质细胞的活性没有差异。总之，这些数据表明炎症和白细胞介素-2 调节视网膜神经节细胞的发育及其轴突在中枢靶区的分布。
