GI Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Toxins (Basel). 2010 Jun;2(6):1318-35. doi: 10.3390/toxins2061318. Epub 2010 Jun 7.
Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology.
志贺毒素（Stx）由侵袭性志贺氏痢疾杆菌血清型 1（S. dysenteriae1）产生，可引起胃肠道和肾脏并发症。人们认为 Stx 是在 S. dysenteriae1 侵入肠细胞后从细胞内释放出来的。然而，关于 Stx 在感染 S. dysenteriae1 的肠细胞内的分布的信息很少。在这里，我们使用肠上皮 T84 细胞来描述类似于 S. dysenteriae1 感染的方面，将 Stx 递送到细胞质中的运输情况进行特征描述。我们发现细胞质中的 Stx 被转运到核仁中。Stx 核仁运动依赖载体和能量。Stx 与体外正常人类肠细胞的核仁结合支持核仁运输在毒素诱导的肠道病理学中的可能作用。
