Abugassa S, Svensson O
Department of General Surgery, Karolinska Institute, Huddinge University, Hospital, Sweden.
Int J Exp Pathol. 1990 Oct;71(5):631-8.
We studied the effects of calciopenia and phosphopenia on longitudinal growth, skeletal mineralization, and development of rickets in young Sprague-Dawley rats. At an age of 21 days, two experimental groups were given diets containing 0.02% calcium or 0.02% phosphorus; otherwise the diets were nutritionally adequate. After 7, 14, and 21 days, five animals from each group were randomly chosen. The animals were anaesthetized and blood samples were drawn for analysis of calcium, phosphorus, and immunoreactive parathyroid hormone, whereupon the animals were killed. Length, weight, and specific weight of the left femur were measured. After 28 days on the respective diet the remaining animals were killed and one proximal tibia from each animal was processed for light microscopy and subjected to stereological analysis. Both experimental groups developed progressive growth retardation, more so the phosphate-depleted group. The calciopenic animals developed severe hypocalcaemia and secondary hyperparathyroidism, whereas the phosphate-depleted animals, in spite of marked secondary hypercalcaemia, had unaltered levels of immunoreactive parathyroid hormone. By 28 days both experimental groups displayed rachitic changes, more pronounced in the phosphate-depleted animals. This paper provides quantitative data demonstrating that calciopenia per se may cause rickets in young rats, but that the rachitic changes in this condition are less severe, and the growth pattern different from those in phosphate depletion.
我们研究了钙缺乏和磷缺乏对幼年斯普拉格-道利大鼠纵向生长、骨骼矿化和佝偻病发展的影响。在21日龄时，两个实验组分别给予含0.02%钙或0.02%磷的饲料；其他方面，饲料营养充足。7天、14天和21天后，每组随机选取5只动物。将动物麻醉后采集血样，用于分析钙、磷和免疫反应性甲状旁腺激素，随后处死动物。测量左股骨的长度、重量和比重。在各自的饲料喂养28天后，处死其余动物，对每只动物的一根近端胫骨进行处理，用于光学显微镜检查并进行体视学分析。两个实验组均出现进行性生长迟缓，磷缺乏组更为明显。钙缺乏的动物出现严重低钙血症和继发性甲状旁腺功能亢进，而磷缺乏的动物尽管有明显的继发性高钙血症，但免疫反应性甲状旁腺激素水平未改变。到28天时，两个实验组均出现佝偻病改变，在磷缺乏的动物中更为明显。本文提供了定量数据，表明钙缺乏本身可能导致幼鼠患佝偻病，但这种情况下的佝偻病改变较轻，且生长模式与磷缺乏时不同。
