Bartels P C, Roijers A F
Clin Chim Acta. 1975 Jun 2;61(2):135-44. doi: 10.1016/0009-8981(75)90307-1.
Parameters which influence the initial rate of formation of phosphomolybdenum blue from phosphate, molybdate, polyvinylpyrrolidone and o-phenylenediamine were investigated. From kinetic data it is concluded that polyvinylpyrrolidone not only acts as a protective colloid, but that it takes part in the reaction and a reaction mechanism is proposed dealing with the stoichiometry of polyvinylpyrrolidone. A rate procedure for the determination of inorganic phosphate in serum and urine, based on this study, is given. Deproteinization of samples is not necessary. The method is rapid requiring one minute for quantitative readings. Because of this short period there is hardly any hydrolysis of organic phosphate esters. As the rate of color development is determined blanks can be omitted. Turbid samples may be analysed with little error.
研究了影响由磷酸盐、钼酸盐、聚乙烯吡咯烷酮和邻苯二胺生成磷钼蓝初始速率的参数。从动力学数据得出结论，聚乙烯吡咯烷酮不仅起保护胶体的作用，而且参与反应，并提出了一个涉及聚乙烯吡咯烷酮化学计量关系的反应机理。基于该研究给出了一种测定血清和尿液中无机磷酸盐的速率方法。样品无需进行脱蛋白处理。该方法快速，定量读数只需一分钟。由于时间短，有机磷酸酯几乎没有水解。由于测定的是显色速率，因此可以省略空白。对浑浊样品进行分析时误差很小。
