Giltay J C, Brinkman H J, Vlekke A, Kiefel V, van Mourik J A, von dem Borne A E
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Br J Haematol. 1990 Aug;75(4):557-60. doi: 10.1111/j.1365-2141.1990.tb07798.x.
To obtain information on the immunological relationship between the endothelial and platelet glycoprotein (GP)Ia-IIa (VLA-2) complex, we studied whether endothelial GPIa-IIa was able to express the platelet GPIa-IIa-associated Br-alloantigen system. Therefore, we tested antisera to both allelic forms of the Br system (Bra and Brb) on platelets (by an assay based on monoclonal antibody-specific immobilization of platelet antigens, MAIPA) and on cultured umbilical vein endothelial cells (by immunoprecipitation experiments) from the same individual. Endothelial cells from a platelet Br(a + b +), and from a platelet Br(a - b +) individual were studied. Our results indicate that endothelial GPIa-IIa is indistinguishable from platelet GPIa-IIa in its ability to express the Bra and Brb alloantigens. The association of Br alloantigens with endothelial GPIa-IIa was confirmed by the results of an assay based on monoclonal antibody-specific immobilization of endothelial antigens (MAIEA). These data further illustrate the structural and immunologic similarity of platelet and endothelial cell GPIa-IIa (VLA-2).
为获取有关内皮细胞糖蛋白（GP）Ia-IIa（VLA-2）复合物与血小板之间免疫关系的信息，我们研究了内皮细胞GPIa-IIa是否能够表达与血小板GPIa-IIa相关的Br同种异体抗原系统。因此，我们使用来自同一受试者的血小板（通过基于血小板抗原单克隆抗体特异性固定的检测方法，即MAIPA）和培养的脐静脉内皮细胞（通过免疫沉淀实验）检测了针对Br系统两种等位基因形式（Bra和Brb）的抗血清。我们研究了来自血小板Br(a + b +)个体以及血小板Br(a - b +)个体的内皮细胞。我们的结果表明，内皮细胞GPIa-IIa在表达Bra和Brb同种异体抗原的能力方面与血小板GPIa-IIa无法区分。基于内皮抗原单克隆抗体特异性固定的检测结果（MAIEA）证实了Br同种异体抗原与内皮细胞GPIa-IIa的关联。这些数据进一步说明了血小板和内皮细胞GPIa-IIa（VLA-2）在结构和免疫方面的相似性。
