[鼻息肉人上皮细胞三种体外原代培养方法的比较研究]
[Comparative study of three primary culture methods of human epithelial cells of nasal polyps in vitro].
作者信息
Wang Mingming, Zhang Guodong, Wang Xiaoting, Li Jianfeng, Wang Haibo
机构信息
Department of Otolaryngology-Head and Neck Surgery, the Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Sep;25(17):796-9.
OBJECTIVE
To compare three primary culture methods of human epithelial cells of nasal polyps in vitro, and explore a suitable method to be used in further study.
METHOD
Shape and appearance of human epithelial cells of nasal polyps were observed, and success ratio and growth curve of primarily cultural were achieved by enzymatic dissociation, isolated cell ABC culture and tissue piece culture method respectively.
RESULT
By comparing achievement ratios, the isolated cell ABC culture method (87.5%) was shown to be superior to enzymatic dissociation (78.13%) and tissue piece culture methods (83.33%), but no statistical significance was found (P>0.05). The growth curves of two isolated cell culture methods were higher than that of tissue piece culture.
CONCLUSION
The isolated cell ABC culture model is more suitable for primary culture due to its faster proliferation, less promiscuity, and more stable and reliable cell supply for nasal polyps research.
目的
比较三种鼻息肉人上皮细胞体外原代培养方法，探索一种适用于进一步研究的方法。
方法
观察鼻息肉人上皮细胞的形态和外观，分别通过酶解、分离细胞ABC培养和组织块培养法获得原代培养的成功率和生长曲线。
结果
通过比较成功率，分离细胞ABC培养法（87.5%）优于酶解（78.13%）和组织块培养法（83.33%），但差异无统计学意义（P>0.05）。两种分离细胞培养方法的生长曲线高于组织块培养。
结论
分离细胞ABC培养模型增殖更快、混杂度更低，为鼻息肉研究提供更稳定可靠的细胞供应，更适合原代培养。
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