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Comparison of mRNA features affecting translation initiation and reinitiation.比较影响翻译起始和重起始的 mRNA 特征。
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3
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4
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6
The location of mRNA in the ribosomal 30S initiation complex; site-directed cross-linking of mRNA analogues carrying several photo-reactive labels simultaneously on either side of the AUG start codon.mRNA在核糖体30S起始复合物中的定位；在AUG起始密码子两侧同时携带多个光反应性标签的mRNA类似物的定点交联。
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7
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本文引用的文献

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Ribosomal proteins of Escherichia coli. II. Proteins from the 30 s subunit.大肠杆菌的核糖体蛋白。II. 来自30S亚基的蛋白。
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Information content of binding sites on nucleotide sequences.核苷酸序列上结合位点的信息内容。
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Posttranscriptional regulatory mechanisms in Escherichia coli.大肠杆菌中的转录后调控机制。
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Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region.劳氏肉瘤病毒gag-pol区域中核糖体移码的信号。
Cell. 1988 Nov 4;55(3):447-58. doi: 10.1016/0092-8674(88)90031-1.
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A persistent untranslated sequence within bacteriophage T4 DNA topoisomerase gene 60.噬菌体T4 DNA拓扑异构酶基因60内一段持续存在的未翻译序列。
Science. 1988 Feb 26;239(4843):1005-12. doi: 10.1126/science.2830666.
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Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot.一种高效冠状病毒核糖体移码信号的表征：RNA假结的要求
Cell. 1989 May 19;57(4):537-47. doi: 10.1016/0092-8674(89)90124-4.
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[Localization of mRNA segments, screened by ribosomes in initiator complexes with the help of a reverse transcription reaction].
Dokl Akad Nauk SSSR. 1989;305(4):1000-3.
8
Mapping the lacZ ribosome binding site by RNA footprinting.通过RNA足迹法绘制lacZ核糖体结合位点图谱。
Biochemistry. 1989 Oct 3;28(20):8067-72. doi: 10.1021/bi00446a015.
9
Extension inhibition analysis of translation initiation complexes.翻译起始复合物的延伸抑制分析
Methods Enzymol. 1988;164:419-25. doi: 10.1016/s0076-6879(88)64058-4.
10
What constitutes the signal for the initiation of protein synthesis on Escherichia coli mRNAs?什么是大肠杆菌信使核糖核酸上蛋白质合成起始的信号？
J Mol Biol. 1988 Nov 5;204(1):79-94. doi: 10.1016/0022-2836(88)90601-8.

起始密码子下游的mRNA序列向单链构象的转变受到起始tRNA结合的强烈促进。

Transition of the mRNA sequence downstream from the initiation codon into a single-stranded conformation is strongly promoted by binding of the initiator tRNA.

作者信息

Balakin A, Skripkin E, Shatsky I, Bogdanov A

机构信息

A.N. Belozersky Laboratory, Moscow State University, U.S.S.R.

出版信息

Biochim Biophys Acta. 1990 Aug 27;1050(1-3):119-23. doi: 10.1016/0167-4781(90)90151-q.

DOI:10.1016/0167-4781(90)90151-q
PMID:2207137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7148821/
Abstract

Using an RNA footprinting technique, accessible sites on the mRNA initiation region bound to the ribosome have been determined. Chemical probing experiments have been done both in the presence and absence of the initiator tRNA with dimethyl sulfate, kethoxal and carbodiimide as reagent probes. As an mRNA, a mini-mRNA containing the initiation region of bacteriophage lambda gene cro has been used. This region is characterized by a long single-stranded Shine-Dalgarno (SD) sequence followed by two hairpin structures of which the first one comprises in its loop the initiation codon. As compared to a free mRNA, the only nucleotides additionally protected in the binary mRNA-ribosome complex have been those which belong to the S-D sequence and the initiation codon. The protection of other nucleotides has not changed. Addition of the initiator RNA results in the modification of nucleotides in the stems of the downstream hairpin structures of the initiation region. This reflects their transition into a single-stranded conformation promoted by tRNA. A possible implication of these findings for the decoding process is discussed.

摘要

利用RNA足迹技术，已确定了与核糖体结合的mRNA起始区域上的可及位点。在有和没有起始tRNA的情况下，分别用硫酸二甲酯、乙二醛和碳二亚胺作为试剂探针进行了化学探测实验。作为mRNA，使用了一种包含噬菌体λ基因cro起始区域的微型mRNA。该区域的特征是有一个长的单链Shine-Dalgarno（SD）序列，其后跟着两个发夹结构，其中第一个发夹结构的环中包含起始密码子。与游离mRNA相比，在二元mRNA-核糖体复合物中额外受到保护的核苷酸仅属于S-D序列和起始密码子。其他核苷酸的保护情况没有变化。添加起始tRNA会导致起始区域下游发夹结构茎中的核苷酸发生修饰。这反映了它们在tRNA促进下转变为单链构象。讨论了这些发现对解码过程可能产生的影响。