劳氏肉瘤病毒gag-pol区域中核糖体移码的信号。
Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region.
作者信息
Jacks T, Madhani H D, Masiarz F R, Varmus H E
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
出版信息
Cell. 1988 Nov 4;55(3):447-58. doi: 10.1016/0092-8674(88)90031-1.
Abstract
The gag-pol protein of Rous sarcoma virus (RSV), the precursor to the enzymes responsible for reverse transcription and integration, is expressed from two genes that lie in different translational reading frames by ribosomal frameshifting. Here, we localize the site of frameshifting and show that the frameshifting reaction is mediated by slippage of two adjacent tRNAs by a single nucleotide in the 5' direction. The gag terminator, which immediately follows the frameshift site, is not required for frameshifting. Other suspected retroviral frameshift sites mediate frameshifting when placed at the end of RSV gag. Mutations in RSV pol also affect synthesis of the gag-pol protein in vitro. The effects of these mutations best correlate with the potential to form an RNA stem-loop structure adjacent to the frameshift site. A short sequence of RSV RNA, 147 nucleotides in length, containing the frameshift site and stem-loop structure, is sufficient to direct frameshifting in a novel genetic context.
摘要
劳氏肉瘤病毒（RSV）的gag-pol蛋白是负责逆转录和整合的酶的前体，它由位于不同翻译阅读框的两个基因通过核糖体移码表达。在这里，我们定位了移码位点，并表明移码反应是由两个相邻的tRNA在5'方向上单个核苷酸的滑动介导的。紧跟移码位点的gag终止子对于移码不是必需的。当置于RSV gag末端时，其他可疑的逆转录病毒移码位点介导移码。RSV pol中的突变也会影响体外gag-pol蛋白的合成。这些突变的影响与在移码位点附近形成RNA茎环结构的潜力最相关。一段长度为147个核苷酸的RSV RNA短序列，包含移码位点和茎环结构，足以在新的遗传背景下指导移码。
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本文引用的文献
1
The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein.烟草蚀纹病毒基因组RNA编码区的核苷酸序列：单一多聚蛋白合成的证据
Virology. 1986 Oct 15;154(1):9-20. doi: 10.1016/0042-6822(86)90425-3.
2
Translation of tobacco rattle virus RNAs in vitro: four proteins from three RNAs.烟草脆裂病毒RNA的体外翻译：三种RNA产生四种蛋白质。
Virology. 1979 Sep;97(2):256-65. doi: 10.1016/0042-6822(79)90337-4.
3
Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.编码Semliki森林病毒膜糖蛋白的cDNA的核苷酸序列。
Nature. 1980 Nov 20;288(5788):236-41. doi: 10.1038/288236a0.
4
Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.辛德毕斯病毒26S mRNA的核苷酸序列及编码的病毒结构蛋白的推导序列。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062-6. doi: 10.1073/pnas.78.4.2062.
5
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.噬菌体T7 DNA的完整核苷酸序列及T7遗传元件的定位
J Mol Biol. 1983 Jun 5;166(4):477-535. doi: 10.1016/s0022-2836(83)80282-4.
6
A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus.一种影响猿猴病毒40大T抗原羧基末端的移码突变导致了一种复制和转化缺陷型病毒。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7065-9. doi: 10.1073/pnas.80.23.7065.
7
Gene fusion vectors based on the gene for staphylococcal protein A.基于葡萄球菌蛋白A基因的基因融合载体。
Gene. 1983 Sep;23(3):369-78. doi: 10.1016/0378-1119(83)90025-2.
8
Ross River virus 26 s RNA: complete nucleotide sequence and deduced sequence of the encoded structural proteins.罗斯河病毒26 s RNA：完整核苷酸序列及推导的编码结构蛋白序列
Virology. 1983 Aug;129(1):170-87. doi: 10.1016/0042-6822(83)90404-x.
9
Nucleotide sequence of Rous sarcoma virus.劳氏肉瘤病毒的核苷酸序列。
Cell. 1983 Mar;32(3):853-69. doi: 10.1016/0092-8674(83)90071-5.
10
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