Bergmann U, Arndt E
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Germany.
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):56-60. doi: 10.1016/0167-4781(90)90141-n.
A small and extremely basic ribosomal protein (HL46e) has been purified from Halobacterium marismortui using reversed-phase high-performance liquid chromatography (HPLC). The amino acid sequence of the protein was determined by automated N-terminal and internal sequence analysis. Comparison of this sequence with other ribosomal protein sequences from eubacteria, archaebacteria and eukaryotes revealed a strong homology to SL46e from Sulfolobus solfataricus, YeaL46 from yeast and RL39 from rat. No significant sequence similarly was found to any eubacterial ribosomal protein so far known. Using a specific oligonucleotide probe the HL46e gene was identified, cloned and the nucleotide sequence including the 5'- and 3'-flanking regions were analysed. The HL46e gene is followed by the gene coding for HL30. A putative halobacterial promoter sequence with the motive 'TTTAAA' has been localized 32 bp upstream of the HL46e gene and a putative terminator sequence localized downstream from the HL30 gene. An equivalent to this HL46e/HL30 operon is apparently not present in Escherichia coli.
一种小的且极其基本的核糖体蛋白（HL46e）已通过反相高效液相色谱法（HPLC）从死海嗜盐菌中纯化出来。该蛋白的氨基酸序列通过自动N端和内部序列分析得以确定。将此序列与来自真细菌、古细菌和真核生物的其他核糖体蛋白序列进行比较，发现它与嗜热栖热菌的SL46e、酵母的YeaL46以及大鼠的RL39具有很强的同源性。到目前为止，尚未发现与任何已知真细菌核糖体蛋白有显著的序列相似性。使用特定的寡核苷酸探针鉴定、克隆了HL46e基因，并分析了包括5'和3'侧翼区域在内的核苷酸序列。HL46e基因之后是编码HL30的基因。一个具有“TTTAAA”基序的假定嗜盐菌启动子序列位于HL46e基因上游32 bp处，一个假定的终止子序列位于HL30基因下游。大肠杆菌中显然不存在与这个HL46e/HL30操纵子等同的序列。
