大鼠核糖体蛋白的一级结构：L27a和L28的氨基酸序列以及S4和S12序列的校正

The primary structure of rat ribosomal proteins: the amino acid sequences of L27a and L28 and corrections in the sequences of S4 and S12.

作者信息

Wool I G, Chan Y L, Paz V, Olvera J

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, IL.

出版信息

Biochim Biophys Acta. 1990 Aug 27;1050(1-3):69-73. doi: 10.1016/0167-4781(90)90143-p.

DOI:10.1016/0167-4781(90)90143-p

PMID:2207170

Abstract

The amino acid sequences of rat ribosomal proteins L27a and L28 were deduced from the sequences of nucleotides in recombinant cDNAs and confirmed from the NH2-terminal amino acid sequences of the proteins. L27a contains 147 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 16 476. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 18-22 copies of the L27a gene. The mRNA for the protein is about 600 nucleotides in length. L27a is homologous to mouse L27a (there are 3 amino acid changes) and to yeast L29. Rat ribosomal protein L28 has 136 amino acids (its NH2-terminal methionine is also processed after translation) and has a molecular weight of 15 707. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 9 or 10 copies of the L28 gene. The mRNA for the protein is about 640 nucleotides in length. L28 contains a possible internal duplication of 9 residues. Corrections are recorded in the sequences reported before for rat ribosomal proteins S4 and S12.

摘要

大鼠核糖体蛋白L27a和L28的氨基酸序列是根据重组cDNA中的核苷酸序列推导出来的，并通过蛋白质的NH2末端氨基酸序列得到证实。L27a含有147个氨基酸（mRNA翻译后NH2末端的甲硫氨酸被去除），分子量为16476。cDNA与核DNA消化产物的杂交表明，L27a基因有18 - 22个拷贝。该蛋白的mRNA长度约为600个核苷酸。L27a与小鼠L27a同源（有3个氨基酸变化），与酵母L29同源。大鼠核糖体蛋白L28有136个氨基酸（其NH2末端的甲硫氨酸在翻译后也被加工），分子量为15707。cDNA与核DNA消化产物的杂交表明，L28基因有9或10个拷贝。该蛋白的mRNA长度约为640个核苷酸。L28含有一个可能的9个残基的内部重复序列。之前报道的大鼠核糖体蛋白S4和S12序列中的修正已记录在案。