大鼠核糖体蛋白S4的一级结构。

The primary structure of rat ribosomal protein S4.

作者信息

Devi K R, Chan Y L, Wool I G

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.

出版信息

Biochim Biophys Acta. 1989 Jul 7;1008(2):258-62. doi: 10.1016/0167-4781(80)90019-6.

DOI:10.1016/0167-4781(80)90019-6

PMID:2660908

Abstract

The amino acid sequence of the rat 40 S ribosomal subunit protein S4 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino-acid sequence of the protein. Ribosomal protein S4 has 282 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 31,841. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 7-11 copies of the S4 gene. The mRNA for the protein is about 1 kb in length. Rat S4 is homologous to Saccharomyces cerevisiae YS6. The protein contains a possible internal duplication of 11 residues.

摘要

大鼠40S核糖体亚基蛋白S4的氨基酸序列是从重组cDNA中的核苷酸序列推导出来的，并通过该蛋白的NH2末端氨基酸序列得到证实。核糖体蛋白S4有282个氨基酸（mRNA翻译后NH2末端的甲硫氨酸被去除），分子量为31,841。cDNA与核DNA消化产物的杂交表明，S4基因有7-11个拷贝。该蛋白的mRNA长度约为1kb。大鼠S4与酿酒酵母YS6同源。该蛋白含有一个可能的11个残基的内部重复序列。