Bohari Siti P M, Hukins David W L, Grover Liam M
School of Mechanical Engineering, University of Birmingham, Edgbaston, UK.
Biomed Mater Eng. 2011;21(3):159-70. doi: 10.3233/BME-2011-0665.
Alginate hydrogels have been used widely in tissue engineering for cell encapsulation for several reasons: low toxicity, the ability to gel under gentle condition and compatibility with cells. In this study, we determined the effect of different concentrations of alginate on encapsulation of 3T3 fibroblast cells at two different cell seeding densities. Live/dead staining and MTT assay were performed at regular intervals up to 4 weeks. A Hoechst 33258 assay was done to validate the MTT results. There were more dead cells on day 1 for the higher concentrations of alginate while at, the lower concentration of alginate, cell proliferation and spheroid formation occurred more quickly. Furthermore, at low cell seeding density, cell proliferation was prolonged compared to the intermediate seeding density. In conclusion, by altering both alginate concentration and cell seeding density, proliferation and spheroid formation can be controlled.
由于具有低毒性、能在温和条件下凝胶化以及与细胞的相容性等几个原因，藻酸盐水凝胶已被广泛用于组织工程中的细胞封装。在本研究中，我们测定了不同浓度的藻酸盐在两种不同细胞接种密度下对3T3成纤维细胞封装的影响。在长达4周的时间内定期进行活/死染色和MTT测定。进行了Hoechst 33258测定以验证MTT结果。对于较高浓度的藻酸盐，第1天死细胞更多，而对于较低浓度的藻酸盐，细胞增殖和球体形成发生得更快。此外，在低细胞接种密度下，与中等接种密度相比，细胞增殖时间延长。总之，通过改变藻酸盐浓度和细胞接种密度，可以控制细胞增殖和球体形成。
