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[Methylation of Eco RI (GAm'ATTC) sequences of bacterial DNA].

作者信息

Lebenka A Iu

出版信息

Biokhimiia. 1990 Jun;55(6):1132-9.

PMID:2207210
Abstract

The methylation of Eco RI (GAm6 ATTC) sequences of DNA of bacteria related to the main branches of their phylogenetic dendrogramme, was studied. It was found that methylation of Eco RI sites is observed in bacteria Caulobacter and in Thermus aquaticus. This finding can be substantiated by the resistance of these DNAs to Eco RI restrictase as well as by the fact that Bam HI fragments of these DNAs cloned within the composition of the vector plasmid pUC8 in E. coli cells contain GAATTC sites.

摘要

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