Kalinin V N, Ovechko N N, Granovskiĭ N N
Vopr Med Khim. 1983 May-Jun;29(3):8-11.
Cytotoxicity of hybrid plasmide pGalI was associated with the genome site measuring for 1.1.mD and flanked by the recognizing sites of Eco RI restrictase. The cytotoxic effect of pGalI might be limited not only by plasmide pMB9 but also by other plasmides constructed on the base of pMBI replicone. Limitation of the cytotoxicity occurred due to a decrease in amount of the pGalI copies. Capacity of the plasmides studied to limit the pGalI cytotoxicity enabled to clone them as cryptic plasmides under conditions of both simultaneous with pGalI transformation of E. coli and the subsequent selection of clones with the Gal+ phenotype.
杂交质粒pGalI的细胞毒性与一个大小为1.1mD且两侧有Eco RI限制性内切酶识别位点的基因组位点相关。pGalI的细胞毒性作用可能不仅受到质粒pMB9的限制，还受到其他基于pMBI复制子构建的质粒的限制。细胞毒性的限制是由于pGalI拷贝数的减少所致。所研究的质粒限制pGalI细胞毒性的能力使得在与pGalI同时转化大肠杆菌以及随后选择具有Gal +表型的克隆的条件下，能够将它们作为隐蔽质粒进行克隆。
