School of Forestry, Northeast Forestry University, Hexing Road 26, Harbin, 150040, Heilongjiang Province, People's Republic of China.
Appl Microbiol Biotechnol. 2012 Jun;94(5):1365-74. doi: 10.1007/s00253-011-3660-1. Epub 2011 Nov 10.
Aerobic granules after 6 months storage were employed in identical sequencing batch reactors (SBRs) using synthetic wastewater to investigate the impacts of different operational strategies on granules' reactivation process. The SBRs were operated under three operational strategies for reactivation of (a) different organic loading rate (OLR); (b) different ammonia concentration; and (c) different shear force (a superficial upflow air velocity). The results indicated that granules after long-term storage could be successfully recovered after 7 days of operation, and the excellent granule reactivation performance was closely related to the operational strategies, since inappropriate operational strategies could cause the outgrowth of filamentous bacteria and granule disintegration. Based on comprehensive comparison of reactivation performance under different operational strategies, the optimal operation strategy for granule reactivation was suggested at OLR of 0.8 kg COD/m(3)/day, ammonia concentration of 15-20 mg/L, and a superficial upflow air velocity of 2.6 cm/s. After 7 days operation under the optimal strategy, the dark brown granules (12 months storage) restored their bioactivities to previous state, in terms of COD removal efficiency (97.44%) and specific oxygen uptake rate (40.63 mg O(2)/g SS h(-1)). The results shed light on the future practical application of stored aerobic granules as bioseed for reactor fast start-up.
采用经过 6 个月储存的好氧颗粒污泥在相同的序批式反应器（SBR）中处理合成废水，以研究不同操作策略对颗粒污泥的恢复过程的影响。SBR 采用三种操作策略进行运行，以恢复（a）不同的有机负荷率（OLR）；（b）不同的氨浓度；和（c）不同的剪切力（表面上流空气速度）。结果表明，经过长期储存的颗粒污泥在经过 7 天的运行后可以成功恢复，并且出色的颗粒污泥恢复性能与操作策略密切相关，因为不适当的操作策略会导致丝状菌的过度生长和颗粒的崩解。基于不同操作策略下的恢复性能的综合比较，建议将 OLR 为 0.8 kg COD/m(3)/day、氨浓度为 15-20 mg/L 和表面上流空气速度为 2.6 cm/s 作为颗粒污泥恢复的最佳操作策略。在最佳策略下运行 7 天后，深褐色颗粒（储存 12 个月）的生物活性得以恢复，COD 去除效率（97.44%）和比需氧量（40.63 mg O(2)/g SS h(-1)）恢复到之前的水平。该结果为储存的好氧颗粒污泥作为反应器快速启动的生物种子的实际应用提供了参考。
