Zhang Linan, Long Bei, Wu Junfeng, Cheng Yuanyuan, Zhang Binchao, Zeng Yu, Huang Sinong, Zeng Mingjing
School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China.
Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology, Mingyue Road, Pingdingshan, 467036, Henan, China.
Heliyon. 2019 Dec 12;5(12):e03023. doi: 10.1016/j.heliyon.2019.e03023. eCollection 2019 Dec.
Aerobic granular sludge (AGS) was imbedded in agar and stored at 4 °C for 30 days, and then the stored granules were recovered in a sequencing batch reactor fed real wastewater within 11 days. Variations in microbial community compositions were investigated during dry storage and recovery of AGS, aiming to elucidate the mechanism of granular stability loss and recovery. The storage and recovery of AGS involved microbial community evolution. The dominant bacterial genera of the mature AGS were (relative abundance of 22.39%), (16.03%) and (11.17%), and those of the stored granules were (26.79%), (12.83%) and (5.69%), respectively. However, the dominant genera were (43.64%), (12.3.6%) and (11.47%) in the recovered AGS. Methanogens were always the dominant archaeal species in mature AGS (93.01%), stored granules (99.99%) and the recovered AGS (94.84%). Facultative anaerobes and anaerobes proliferated and dominated in the stored granules, and their metabolic activities gradually led to granular structure destruction and property deterioration. However, the stored granules served as carriers for the microbes originated from the real septic tank wastewater during recovery. They proliferated rapidly and secreted a large number of extracellular polymeric substances which helped to recover the granular structure in 11 days.
好氧颗粒污泥（AGS）被包埋在琼脂中，于4℃下储存30天，然后将储存的颗粒在序批式反应器中回收，在11天内加入实际废水。研究了AGS在干燥储存和回收过程中微生物群落组成的变化，旨在阐明颗粒稳定性丧失和恢复的机制。AGS的储存和回收涉及微生物群落的演变。成熟AGS的优势细菌属为（相对丰度22.39%）、（16.03%）和（11.17%），储存颗粒的优势细菌属分别为（26.79%）、（12.83%）和（5.69%）。然而，回收的AGS中的优势属为（43.64%）、（12.3.6%）和（11.47%）。产甲烷菌始终是成熟AGS（93.01%）、储存颗粒（99.99%）和回收的AGS（94.84%）中的优势古菌物种。兼性厌氧菌和厌氧菌在储存颗粒中增殖并占主导地位，它们的代谢活动逐渐导致颗粒结构破坏和性能恶化。然而，储存颗粒在回收过程中作为来自实际化粪池废水的微生物的载体。它们迅速增殖并分泌大量胞外聚合物，有助于在11天内恢复颗粒结构。
