Institute for Research in Biomedicine, Baldiri Reixac 10, Barcelona E-08028, Spain; E-Mail:
Int J Mol Sci. 2011;12(10):7238-49. doi: 10.3390/ijms12107238. Epub 2011 Oct 24.
An oligodeoxynucleotide hairpin containing a photolabile 2-nitrobenzyl group in the loop and terminated with a thiol function was prepared. The photocleavage of such a hairpin on gold yields a surface activated with a single stranded oligonucleotide which can be utilised to direct the assembly of nanoparticles conjugated with a complementary strand. Analysis of photocleaved surfaces gives nanoparticle coverage one order of magnitude higher than nonphotocleaved surfaces. This illustrates the ability of photocleavable hairpins to direct the assembly of nanomaterials on conducting materials. The conjugation of the photocleavable hairpin to a gold nanoparticle allows the observation of intermolecular interactions between hairpins linked in different nanoparticles, by comparing the thermal dissociations of a hairpin-nanoparticle conjugates at 260 nm and 520 nm. We have also shown that it is possible to permanently alter the physiochemical properties of DNA-nanoparticles by the introduction of a photocleavable group. Indeed for the first time it has been shown that by exposure to UV light the disassembly of nanoparticle aggregates can be induced.
制备了一种含有光解 2-硝基苄基基团的发夹状寡核苷酸，其环中含有一个硫醇功能。在金表面上，这种发夹的光解会产生一个表面，该表面被激活为单链寡核苷酸，可用于指导与互补链连接的纳米粒子的组装。对光解表面的分析表明，纳米粒子的覆盖率比非光解表面高一个数量级。这说明了光解发夹引导纳米材料在导电材料上组装的能力。将光解发夹与金纳米粒子连接，通过比较在 260nm 和 520nm 下发夹-纳米粒子缀合物的热解，观察连接在不同纳米粒子中的发夹之间的分子间相互作用。我们还表明，通过引入光解基团，可以永久改变 DNA-纳米粒子的物理化学性质。事实上，这是首次表明通过暴露于紫外光，可以诱导纳米粒子聚集体的解组装。
