Claridge Shelley A, Liang Huiyang W, Basu S Roger, Fréchet Jean M J, Alivisatos A Paul
Department of Chemistry, Berkeley, CA 94720, USA.
Nano Lett. 2008 Apr;8(4):1202-6. doi: 10.1021/nl0802032. Epub 2008 Mar 11.
Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (> or =20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.
通过阴离子交换高效液相色谱法已纯化出了离散的DNA-金纳米颗粒复合物，其中5纳米和20纳米的金颗粒所结合的DNA长度短至15个碱基。由短DNA（<40个碱基）和大金颗粒（≥20纳米）组成的复合物很难通过其他方法纯化，并且是等离子体耦合实验的潜在底物。通过将互补复合物杂交形成离散结构来证明复合物的纯度，这些结构通过透射电子显微镜观察。
