Williams M E, Westermann C D, Swerdlow S H
Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
Blood. 1990 Oct 1;76(7):1387-91.
Centrocytic lymphomas are defined in the Kiel classification as B-cell lymphomas composed exclusively of cells resembling cleaved follicular center cells (FCC). These lymphomas have been shown to be histologically, immunophenotypically, and clinically distinct from other cleaved FCC lymphomas. DNA from 18 centrocytic lymphomas (14 patients) was analyzed using Southern blotting and probes for immunoglobulin heavy (JH) and kappa light chain (JK) joining gene, T-cell receptor beta chain constant gene (CB), bcl-1, bcl-2, and c-myc gene rearrangements. All of the lymphomas had JH and JK rearrangements, confirming their B-cell origin. None of the specimens had detectable CB, bcl-2, or c-myc rearrangements. However, 4 of 14 patients (28.6%) had rearrangement of the chromosome 11 bcl-1 locus. Therefore, centrocytic lymphomas are genotypically distinguishable from the majority of other small cleaved FCC lymphomas by their lack of demonstrable bcl-2 rearrangements. This supports the distinct nature of centrocytic lymphomas and suggests the lack of importance for the putative oncogene bcl-2 in these cases. Furthermore, the frequent rearrangement of bcl-1 suggests a possible role for this locus in the pathogenesis of at least some centrocytic lymphomas.
在基尔分类中，中心细胞性淋巴瘤被定义为完全由类似于裂核滤泡中心细胞（FCC）的细胞组成的B细胞淋巴瘤。这些淋巴瘤在组织学、免疫表型和临床上已被证明与其他裂核FCC淋巴瘤不同。使用Southern印迹法以及免疫球蛋白重链（JH）和κ轻链（JK）连接基因、T细胞受体β链恒定基因（CB）、bcl-1、bcl-2和c-myc基因重排的探针，对18例中心细胞性淋巴瘤（14例患者）的DNA进行了分析。所有淋巴瘤均有JH和JK重排，证实其起源于B细胞。所有标本均未检测到CB、bcl-2或c-myc重排。然而，14例患者中有4例（28.6%）存在11号染色体bcl-1位点重排。因此，中心细胞性淋巴瘤在基因分型上与大多数其他小裂核FCC淋巴瘤不同，因为它们缺乏可证实的bcl-2重排。这支持了中心细胞性淋巴瘤的独特性质，并表明在这些病例中假定的癌基因bcl-2不重要。此外，bcl-1的频繁重排表明该位点在至少一些中心细胞性淋巴瘤的发病机制中可能起作用。
