Ott M M, Ott G, Kuse R, Porowski P, Gunzer U, Feller A C, Müller-Hermelink H K
Institute of Pathology, University of Würzburg, Germany.
Histopathology. 1994 Apr;24(4):329-34. doi: 10.1111/j.1365-2559.1994.tb00533.x.
Ten cases of classic centrocytic lymphoma as defined in the Kiel classification system were investigated for their immunophenotype, their proliferation activity and by means of molecular diagnostics. The findings were compared to those obtained from a group of nine cases of anaplastic centrocytic lymphoma. Both groups showed virtually identical immunohistochemical characteristics with positivity for CD5 and negativity for CD10 and CD23. In the group of anaplastic centrocytic lymphoma, there were considerably higher proliferation indices as documented by staining for the Ki-67 antigen, up to 80% of the tumour cells being positive. Moreover, the cases of anaplastic centrocytic lymphoma had bcl-1 gene rearrangements in eight out of nine cases compared with three out of 10 cases of classic centrocytic lymphoma. DNA analysis was not able to detect bcl-2 gene rearrangement in any case, pointing to a difference compared with lymphomas of germinal centre origin. The coincidence of anaplastic and sometimes blast-like morphology of the tumour cells, high proliferation index and a rearranged bcl-1 gene in nearly all cases of anaplastic centrocytic lymphoma support their classification as high-grade malignant variants of centrocytic lymphoma and suggest a possible role for the bcl-1 locus not only in the origin but also in the progression of centrocytic lymphomas.
我们对根据基尔分类系统定义的10例经典中心细胞性淋巴瘤进行了免疫表型、增殖活性及分子诊断方面的研究。并将研究结果与9例间变性中心细胞性淋巴瘤病例的结果进行了比较。两组的免疫组化特征几乎相同，均表现为CD5阳性、CD10和CD23阴性。在间变性中心细胞性淋巴瘤组中，通过Ki-67抗原染色记录的增殖指数显著更高，高达80%的肿瘤细胞呈阳性。此外，9例间变性中心细胞性淋巴瘤中有8例存在bcl-1基因重排，而10例经典中心细胞性淋巴瘤中只有3例出现该情况。DNA分析在任何病例中均未检测到bcl-2基因重排，这表明与生发中心来源的淋巴瘤存在差异。间变性中心细胞性淋巴瘤几乎所有病例中肿瘤细胞呈间变性甚至有时呈母细胞样形态、高增殖指数以及bcl-1基因重排，这些支持将其归类为中心细胞性淋巴瘤的高级别恶性变体，并提示bcl-1基因座不仅在中心细胞性淋巴瘤的起源中，而且在其进展中可能发挥作用。
