Shanghai Renji Hospital, Jiaotong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, China.
PLoS One. 2011;6(11):e27038. doi: 10.1371/journal.pone.0027038. Epub 2011 Nov 2.
BACKGROUND/AIMS: Regulatory T cells (Tregs) and natural killer T (NKT) cells are two distinct lymphocyte subsets that independently regulate hepatic adaptive and innate immunity, respectively. In the current study, we examine the interaction between Tregs and NKT cells to understand the mechanisms of cross immune regulation by these cells.
The frequency and function of Tregs were evaluated in wild type and NKT cell deficient (CD1dko) mice. In vitro lymphocyte proliferation and apoptosis assays were performed with NKT cells co-cultured with Tregs. The ability of Tregs to inhibit NKT cells in vivo was examined by adoptive transfer of Tregs in a model of NKT cell mediated hepatitis.
CD1dko mice have a significant reduction in hepatic Tregs. Although, the Tregs from CD1dko mice remain functional and can suppress conventional T cells, their ability to suppress activation induced NKT cell proliferation and to promote NKT cell apoptosis is greatly diminished. These effects are CD1d dependent and require cell to cell contact. Adoptive transfer of Tregs inhibits NKT cell-mediated liver injury.
NKT cells promote Tregs, and Tregs inhibit NKT cells in a CD1d dependent manner requiring cell to cell contact. These cross-talk immune regulations provide a linkage between innate and adaptive immunity.
背景/目的：调节性 T 细胞（Tregs）和自然杀伤 T（NKT）细胞是两个不同的淋巴细胞亚群，分别独立调节肝脏适应性和固有免疫。在本研究中，我们研究了 Tregs 和 NKT 细胞之间的相互作用，以了解这些细胞的交叉免疫调节机制。
在野生型和 NKT 细胞缺陷（CD1dko）小鼠中评估 Tregs 的频率和功能。用 Tregs 共培养进行体外淋巴细胞增殖和凋亡实验。通过在 NKT 细胞介导的肝炎模型中过继转移 Tregs，检查 Tregs 在体内抑制 NKT 细胞的能力。
CD1dko 小鼠肝脏 Tregs 明显减少。尽管 CD1dko 小鼠的 Tregs 仍具有功能且能抑制常规 T 细胞，但它们抑制活化诱导的 NKT 细胞增殖和促进 NKT 细胞凋亡的能力大大降低。这些作用依赖于 CD1d，需要细胞间接触。过继转移 Tregs 可抑制 NKT 细胞介导的肝损伤。
NKT 细胞促进 Tregs，Tregs 以 CD1d 依赖的方式抑制 NKT 细胞，需要细胞间接触。这些交叉免疫调节提供了固有免疫和适应性免疫之间的联系。
