Shindo N, Yamauchi N, Murayama K, Fairbrother A, Korlik S
Division of Biochemical Analysis, Juntendo University, School of Medicine, Tokyo, Japan.
Biomed Chromatogr. 1990 Jul;4(4):171-4. doi: 10.1002/bmc.1130040413.
A very sensitive, selective and simultaneous method for the analysis of salivary steroid hormones was examined by discharge-assisted thermospray liquid chromatography mass spectrometry (plasmaspray LC/MS). Plasmaspray LC/MS gave [M + H]+ or [MH - H2O]+ as a predominant ion in most of the steroids in this work and in some cases fragment ions were also observed. Eight salivary steroid hormones, pregnenolone, 17-hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol, aldosterone, and testosterone were identified within 10 minutes using the selected ion monitoring technique in conjunction with plasmaspray LC/MS. Progesterone, 17-hydroxyprogesterone, cortisol, aldosterone and testosterone were detected in 1 mL of saliva from a normal child and two patients with congenital adrenal hyperplasia.
采用放电辅助热喷雾液相色谱质谱联用仪（等离子体喷雾液相色谱/质谱联用仪）对一种用于分析唾液中甾体激素的非常灵敏、选择性强且能同时进行分析的方法进行了研究。在本研究中，等离子体喷雾液相色谱/质谱联用仪在大多数甾体化合物中给出了[M + H]+或[MH - H₂O]+作为主要离子，在某些情况下还观察到了碎片离子。使用选定离子监测技术结合等离子体喷雾液相色谱/质谱联用仪，在10分钟内鉴定出了8种唾液甾体激素，即孕烯醇酮、17-羟孕烯醇酮、孕酮、17-羟孕酮、11-脱氧皮质醇、皮质醇、醛固酮和睾酮。在一名正常儿童以及两名先天性肾上腺皮质增生症患者的1 mL唾液中检测到了孕酮、17-羟孕酮、皮质醇、醛固酮和睾酮。
