通过棉子糖-琼脂糖凝胶6B亲和层析从海洋海绵锚状德斯马海绵中分离一种凝集素。

Isolation of a lectin from the marine sponge Desmapsama anchorata by affinity chromatography on raffinose-sepharose 6B.

作者信息

Atta A M, Menezes E P, Peixinho S, Sousa-Atta M L

机构信息

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, Brasil.

出版信息

Braz J Med Biol Res. 1990;23(2):191-4.

PMID:2207445

Abstract

A mitogenic lectin for human lymphocytes is present in the marine sponge Desmapsama anchorata. The protein hemagglutinates red blood cells irrespective of ABO group antigens. We now report the isolation of this lectin, by affinity chromatography on a column of raffinose conjugated to epoxy-activated Sepharose 6B, in 8.3% yield and with a purification index of 27 based on hemagglutinating activity. The isolated lectin is a glycoprotein with two subunits with molecular weights of about 18 and 36 kDa which display carbohydrate combining sites of similar specificities and can be associated in different forms.

摘要

海洋海绵锚状Desmapsama中存在一种对人淋巴细胞有促有丝分裂作用的凝集素。该蛋白能凝集红细胞，而与ABO血型抗原无关。我们现在报告通过在与环氧活化的琼脂糖凝胶6B偶联的棉子糖柱上进行亲和层析分离这种凝集素，产率为8.3%，基于血凝活性的纯化指数为27。分离出的凝集素是一种糖蛋白，有两个亚基，分子量约为18 kDa和36 kDa，它们具有相似特异性的碳水化合物结合位点，并且可以以不同形式结合。