Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia.
Reprod Toxicol. 2012 Jan;33(1):20-6. doi: 10.1016/j.reprotox.2011.10.009. Epub 2011 Oct 29.
The purpose of the present study was to determine changes in plasma paraoxonase-1 activity (an indicator of paraoxonase phenotype) throughout normal pregnancy and its relationship with maternal oxidative stress status. The frequencies of the paraoxonase-1 phenotype in the studied population were determined using a two-substrate (paraoxon/diazoxon) activity method. As a parameter of oxidative stress status we measured the redox balance. Paraoxonase-1 activity significantly decreased at gestational week 32. In addition, the lipid profile was more atherogenic. Redox balance was significantly increased across gestational weeks. There were independent direct associations between maternal smoking habits before pregnancy, glucose concentrations and redox balance with PON1 activity in the third trimester. This study shows that pregnancy is followed by a decrease in PON1 activity and increased risk for development of cardiovascular diseases. We conclude that changes in paraoxonase-1 status during pregnancy are associated with maternal oxidative stress status and smoking habits.
本研究旨在确定血浆对氧磷酶-1 活性（对氧磷酶表型的指标）在正常妊娠过程中的变化及其与母体氧化应激状态的关系。研究人群中对氧磷酶-1 表型的频率是使用双底物（对氧磷/二嗪肟）活性方法确定的。作为氧化应激状态的参数，我们测量了氧化还原平衡。在妊娠第 32 周时，对氧磷酶-1 活性显著降低。此外，血脂谱更具动脉粥样硬化性。氧化还原平衡在整个孕周期中显著增加。在妊娠晚期，母体在怀孕前的吸烟习惯、血糖浓度和氧化还原平衡与 PON1 活性之间存在独立的直接关联。本研究表明，妊娠后 PON1 活性降低，患心血管疾病的风险增加。我们得出结论，妊娠期间对氧磷酶-1 状态的变化与母体氧化应激状态和吸烟习惯有关。
