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用于三维光激活定位成像的光子高效双螺旋点扩散函数显微镜

Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging.

作者信息

Grover Ginni, Quirin Sean, Fiedler Callie, Piestun Rafael

机构信息

Department of Electrical, Computer, and Energy Engineering, University of Colorado, Boulder, CO 80309, USA.

出版信息

Biomed Opt Express. 2011 Nov 1;2(11):3010-20. doi: 10.1364/BOE.2.003010. Epub 2011 Oct 5.

DOI:10.1364/BOE.2.003010
PMID:22076263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3207371/
Abstract

We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range.

摘要

我们展示了一种基于双螺旋点扩散函数（DH-PSF）的三维（3D）显微镜，该显微镜使用灰度光刻制造的相位掩膜实现了高效的光子收集。与当前的液晶空间光调制器实现方式相比，使用该相位掩膜的系统效率提高了一倍多。我们展示了相位掩膜DH-PSF显微镜在扩展轴向范围内用于三维光激活定位显微镜（PM-DH-PALM）的情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/4794bb494d25/boe-2-11-3010-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/a400575cfdc9/boe-2-11-3010-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/24d379614449/boe-2-11-3010-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/510f4f2ee972/boe-2-11-3010-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/a454cdfdcd30/boe-2-11-3010-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/af529cf7467e/boe-2-11-3010-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/f77a5bb3aaa5/boe-2-11-3010-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/37b26b199843/boe-2-11-3010-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/4794bb494d25/boe-2-11-3010-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/a400575cfdc9/boe-2-11-3010-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/24d379614449/boe-2-11-3010-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/510f4f2ee972/boe-2-11-3010-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/a454cdfdcd30/boe-2-11-3010-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/af529cf7467e/boe-2-11-3010-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/f77a5bb3aaa5/boe-2-11-3010-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/37b26b199843/boe-2-11-3010-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1c/3207371/4794bb494d25/boe-2-11-3010-g008.jpg

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