Nishikaku Angela Satie, Molina Raphael Fagnani Sanchez, Albe Bernardo Paulo, Cunha Cláudia da Silva, Scavone Renata, Pizzo Célia Regina Pinto, de Camargo Zoilo Pires, Burger Eva
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
FEMS Immunol Med Microbiol. 2011 Nov;63(2):281-8. doi: 10.1111/j.1574-695X.2011.00851.x.
The important role of interferon-gamma (IFN-γ) in protective immunity in mycosis is well established, except for its participation in fungal granulomas. Herein, we employ immunohistochemical reactions to describe the in situ localization of IFN-γ in granulomas of susceptible (B10.A) and resistant (A/J) mice to infection with Paracoccidioides brasiliensis (Pb). After infection with the highly virulent Pb18, IFN-γ-positive lymphomononuclear cells were localized mainly at the periphery of granulomas in both mouse strains. The numbers of positive cells found in compact granulomas of A/J mice increased significantly from 15 to 120 days postinfection. At this time, significantly more positive cells were detected in the compact granulomas of resistant mice than in the loose, multifocal lesions of the susceptible ones. In infection with the slightly virulent Pb265, the same pattern of IFN-γ localization was found as in Pb18 infection, but there was decreased staining at 120 days due to the presence of only residual lesions in both mouse strains. The marked IFN-γ staining observed in the granulomas of resistant mice at the later stage of Pb infection confirms its importance in fungal dissemination control, and suggests a contribution to the development of paracoccidioidal granuloma.
除了参与真菌性肉芽肿形成外，干扰素-γ（IFN-γ）在真菌病保护性免疫中的重要作用已得到充分证实。在此，我们采用免疫组化反应来描述IFN-γ在巴西副球孢子菌（Pb）感染的易感（B10.A）和抗性（A/J）小鼠肉芽肿中的原位定位。用高毒力的Pb18感染后，IFN-γ阳性淋巴单核细胞主要定位于两种小鼠品系肉芽肿的周边。在感染后15至120天，A/J小鼠致密肉芽肿中发现的阳性细胞数量显著增加。此时，抗性小鼠致密肉芽肿中检测到的阳性细胞明显多于易感小鼠疏松的多灶性病变中的阳性细胞。用弱毒力的Pb265感染时，IFN-γ的定位模式与Pb18感染时相同，但由于两种小鼠品系在120天时仅存在残留病变，染色有所减弱。在Pb感染后期抗性小鼠肉芽肿中观察到的明显IFN-γ染色证实了其在控制真菌播散中的重要性，并提示其对副球孢子菌性肉芽肿的形成有一定作用。
