Singer-Vermes L M, Burger E, Franco M F, Di-Bacchi M M, Mendes-Giannini M J, Calich V L
Department of Immunology, ICB, University of São Paulo, Brazil.
J Med Vet Mycol. 1989;27(2):71-82.
In this investigation the pathogenicity and immunogenicity of seven isolates of Paracoccidioides brasiliensis were compared. The pathogenicity of each isolate was determined by 50% lethal dose estimations and histopathology analysed during a 24-week-period. Four basic patterns of virulence could be defined after intraperitoneal infection of susceptible, genetically homogenous, B10.A mice, namely, slightly virulent (isolates Pb 265 and IVIC Pb 267), intermediate (isolates Pb 192, IVIC Pb 9 and Pb SN), virulent (isolate Pb 2052) and highly virulent (isolate Pb 18). The granulomas induced by the individual isolates were similar although the evolution of inflammation and the organs affected varied according to the isolate used. The immunogenicity of each P. brasiliensis isolate was evaluated by measuring IgG titres with an ELISA method. The intermediate and the slightly virulent isolates induced weak antibody production whereas isolates Pb 18 and Pb 2052 induced stronger specific humoral responses. Differences in the kinetics of antibody production elicited by the different fungal isolates were also observed.
在本研究中，对7株巴西副球孢子菌的致病性和免疫原性进行了比较。通过50%致死剂量评估确定每株菌株的致病性，并在24周期间分析组织病理学。在对易感的、基因同质的B10.A小鼠进行腹腔感染后，可以确定四种基本的毒力模式，即轻度毒力（菌株Pb 265和IVIC Pb 267）、中度毒力（菌株Pb 192、IVIC Pb 9和Pb SN）、毒力强（菌株Pb 2052）和高毒力（菌株Pb 18）。尽管炎症的演变和受影响的器官因所用菌株而异，但各菌株诱导的肉芽肿相似。通过ELISA方法测量IgG滴度来评估每株巴西副球孢子菌的免疫原性。中度毒力和轻度毒力菌株诱导的抗体产生较弱，而菌株Pb 18和Pb 2052诱导较强的特异性体液反应。还观察到不同真菌菌株引发的抗体产生动力学差异。
