Faculty of Veterinary Medicine, Laboratory of Animal Pathology, University of São Paulo State, Araçatuba, São Paulo, Brazil.
Vet Comp Oncol. 2011 Dec;9(4):251-9. doi: 10.1111/j.1476-5829.2010.00260.x. Epub 2011 Feb 21.
Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.
犬传染性性病肿瘤（CTVT）是一种通过与受伤的皮肤和/或粘膜组织直接接触在健康犬之间传播的肿瘤。本研究旨在鉴定 TP53 基因、信使 RNA（mRNA）以及在不同进化阶段的 13 个 CTVT 样本组织切片中 p53、Bcl-2 和 p63 蛋白的表达。使用原位杂交（ISH）和原位逆转录聚合酶链反应（RT-PCR）检测，结果显示 92.3%的样本存在 TP53 的 DNA 及其相应的 mRNA。我们在不同程度的细胞样本中检测到 p53、p63 和 Bcl-2 蛋白。此外，46%的样本处于进展期，54%处于消退期。这是对这些蛋白的首次描述，需要对它们在 CTVT 细胞中的作用进行详细研究，以验证这些细胞如何发生凋亡。
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