Setthawongsin Chanokchon, Tangkawattana Sirikachorn, Rungsipipat Anudep, Techangamsuwan Somporn
Companion Animal Cancer Research Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, KhonKaen University, KhonKaen, Thailand.
Front Vet Sci. 2019 Apr 9;6:104. doi: 10.3389/fvets.2019.00104. eCollection 2019.
Interferons (IFNs), signaling proteins produced by host cells, are secreted in response to pathogen activity as well as to tumor cells, and display antiviral, antiproliferative, and immunomodulatory effects. Recombinant feline interferon omega (rFeIFN-ω) has growth inhibition activities on various canine and feline tumor cell lines. Canine transmissible venereal tumor (CTVT) is used as an animal model for immunotherapy due to its specific growth phase. Previous studies have usually focused on the interaction between tumor infiltrating lymphocytes (TILs) and CTVT cells. However, the specific effects of rFeIFN-ω on CTVT cells remains poorly defined. The aims of this study, therefore, were to evaluate the effect of rFeIFN-ω on primary CTVT cells and to study the mRNA expression of apoptotic genes and drug resistance genes. Purified CTVT cells were treated with various concentrations of rFeIFN-ω and the viability of the cultured cells was ascertained at 24, 48, and 72 h post treatment (hpt) and a dose-response curve plotted. The mRNA expression of apoptotic ( and ) and drug resistance ( and ) genes was performed by reverse transcription quantitative real-time PCR at 72 hpt. rFeIFN-ω displayed an effect against CTVT cell viability, which decreasing viability in a dose-dependent manner within 72 hpt. The relative mRNA expression of was upregulated only at a rFeIFN-ω concentration of 10 IU/100 μl. However, higher concentrations of rFeIFN-ω gave a higher level of relative mRNA expression of transporter gene. This study provided the information of effect of rFeIFN-ω on CTVT cell viability in a dose dependent manner, as well as, the alteration of and gene expression after treatment. These results encourage future studies to evaluate the potential efficacy of this treatment in CTVT cases.
干扰素（IFNs）是宿主细胞产生的信号蛋白，可响应病原体活性以及肿瘤细胞而分泌，并具有抗病毒、抗增殖和免疫调节作用。重组猫ω干扰素（rFeIFN-ω）对多种犬类和猫类肿瘤细胞系具有生长抑制活性。犬传染性性病肿瘤（CTVT）由于其特定的生长阶段，被用作免疫治疗的动物模型。以往的研究通常集中在肿瘤浸润淋巴细胞（TILs）与CTVT细胞之间的相互作用。然而，rFeIFN-ω对CTVT细胞的具体作用仍不清楚。因此，本研究的目的是评估rFeIFN-ω对原发性CTVT细胞的作用，并研究凋亡基因和耐药基因的mRNA表达。用不同浓度的rFeIFN-ω处理纯化的CTVT细胞，并在处理后24、48和72小时（hpt）确定培养细胞的活力，并绘制剂量反应曲线。在72 hpt时，通过逆转录定量实时PCR检测凋亡基因（和）和耐药基因（和）的mRNA表达。rFeIFN-ω对CTVT细胞活力有影响，在72 hpt内以剂量依赖方式降低活力。仅在rFeIFN-ω浓度为10 IU/100μl时，的相对mRNA表达上调。然而，更高浓度的rFeIFN-ω使转运蛋白基因的相对mRNA表达水平更高。本研究提供了rFeIFN-ω对CTVT细胞活力呈剂量依赖性影响的信息，以及处理后和基因表达的变化。这些结果鼓励未来的研究评估这种治疗方法在CTVT病例中的潜在疗效。
