两种不同提取方法的桃叶珊瑚叶提取物抗动脉粥样硬化作用比较。

Comparison of anti-atherosclerotic effects of two different extracts from leaves of Mallotus furetianus.

机构信息

School of Pharmacy, Hainan Medical College, Haikou 571101, P. R. China.

出版信息

Asian Pac J Trop Med. 2011 Nov;4(11):878-82. doi: 10.1016/S1995-7645(11)60212-5.

DOI:10.1016/S1995-7645(11)60212-5

PMID:22078950

Abstract

OBJECTIVE

To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus furetianus by using rat model of atherosclerosis.

METHODS

The air-dried powdered Mallotus furetianus leaves were extracted with ethanol and then evaporated. The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H2O (50:50, 100:0, v/v) and two fractions, Mallotus furetianus A (Mf A) and Mallotus furetianus B (Mf B) were obtained. Rats were divided into control, atherosclerosis and vitamin E, Mf A and Mf B treated groups. Atherosclerotic model was established by administering a loading dose of vitamin D3 and feeding standard diet enriched with 2% cholesterol, 0.5% porcine cholate, 0.2% methimazole, 5% sugar, 10% pork fat. Vitamin E (0.20 g/kg), Mf A (0.053 g/kg), Mf B (0.057 g/kg) (with the potential) were administered to interfere with the development of atherosclerosis. After 9 weeks, rats were sacrificed and the blood lipid as well as composition of bile was examined. In addition, the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.

RESULTS

Atherosclerosis model was successfully established, administration of vitamin E, Mf A and Mf B increased excretion of total bilirubin in bile, decreased triglyeride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) level, enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood, improved histological changes and diminished intima-media thickness ratio of thoracic aorta in atherosclerotic rats. As for the difference in anti-atherosclerotic effects betweenMf A and Mf B, Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.

CONCLUSIONS

The two different extracts, Mf A and Mf B can prevent the development of atherosclerosis, In detail, Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC level in atherosclerotic rats.

摘要

目的

通过建立动脉粥样硬化大鼠模型，比较不同提取方法得到的算盘子叶提取物的抗动脉粥样硬化作用。

方法

将干燥的算盘子叶粉末用乙醇提取并浓缩，然后通过 Diaion HP-20 CC 柱层析，用甲醇和水（50:50、100:0，v/v）进行梯度洗脱，得到两个部位，分别命名为算盘子叶 A（Mf A）和算盘子叶 B（Mf B）。将大鼠分为对照组、动脉粥样硬化模型组和维生素 E、Mf A、Mf B 治疗组。采用大剂量维生素 D3 灌胃联合普通饲料喂养（添加 2%胆固醇、0.5%猪胆盐、0.2%甲巯咪唑、5%蔗糖、10%猪油）的方法建立动脉粥样硬化模型。维生素 E（0.20 g/kg）、Mf A（0.053 g/kg）、Mf B（0.057 g/kg）（以有效剂量计）进行干预。9 周后处死大鼠，检测血脂和胆汁成分，取胸主动脉观察组织学变化和内膜中层厚度比。

结果

成功建立了动脉粥样硬化模型，维生素 E、Mf A、Mf B 干预后，大鼠胆汁总胆红素排泄量增加，三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平降低，高密度脂蛋白胆固醇与 LDL-C 比值升高，改善了动脉粥样硬化大鼠的组织学变化，降低了胸主动脉内膜中层厚度比。Mf A 和 Mf B 抗动脉粥样硬化作用的差异表现为，Mf A 降低 TG 作用更强，Mf B 降低 TC 作用更明显。