Academic Unit of Molecular Medicine/Rheumatology, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom.
Biomaterials. 2012 Feb;33(4):1007-16. doi: 10.1016/j.biomaterials.2011.10.042. Epub 2011 Nov 9.
Glycosaminoglycans play an important role in tissue organisation through interactions with a diverse range of proteins, growth factors and other chemokines. In this report, we demonstrate the GAG-binding 'fingerprint' of two important GAG-binding proteins - osteoprotogerin and TIMP-3. The technique uses a straightforward method for attaching GAGs to assay surfaces in a non-covalent manner using plasma polymerization that leaves the adsorbed GAG able to participate in subsequent ligand binding. We show that OPG and TIMP-3 bind preferentially to different GAGs in a simple ELISA and that this binding does not correlate directly with simple GAG properties such as degree of sulfation. The methods outlined in this report can be easily applied to tissue engineering scaffolds in order to exploit the potential of surface-bound GAGs in influencing the structure of engineered tissues.
糖胺聚糖通过与多种蛋白质、生长因子和其他趋化因子相互作用，在组织组织中发挥重要作用。在本报告中，我们展示了两种重要的糖胺聚糖结合蛋白——骨保护素和 TIMP-3 的 GAG 结合“指纹”。该技术使用一种简单的方法，通过等离子体聚合以非共价方式将 GAG 附着到检测表面上，使吸附的 GAG 能够参与随后的配体结合。我们表明，OPG 和 TIMP-3 在简单的 ELISA 中优先结合不同的 GAG，并且这种结合与简单的 GAG 特性（如硫酸化程度）没有直接关系。本报告中概述的方法可以很容易地应用于组织工程支架，以利用表面结合的 GAG 对工程组织结构的影响潜力。
