Guz Wiesław, Podgórski Rafał, Aebisher David, Truszkiewicz Adrian, Machorowska-Pieniążek Agnieszka, Cieślar Grzegorz, Kawczyk-Krupka Aleksandra, Bartusik-Aebisher Dorota
Department of Diagnostic Imaging and Nuclear Medicine, Medical College of the University of Rzeszow, 35-310 Rzeszów, Poland.
Department of Biochemistry and General Chemistry, Medical College of the University of Rzeszow, 35-310 Rzeszów, Poland.
Int J Mol Sci. 2024 Mar 5;25(5):3009. doi: 10.3390/ijms25053009.
The aim of this work was to use and optimize a 1.5 Tesla magnetic resonance imaging (MRI) system for three-dimensional (3D) images of small samples obtained from breast cell cultures in vitro. The basis of this study was to design MRI equipment to enable imaging of MCF-7 breast cancer cell cultures (about 1 million cells) in 1.5 and 2 mL glass tubes and/or bioreactors with an external diameter of less than 20 mm. Additionally, the development of software to calculate longitudinal and transverse relaxation times is described. Imaging tests were performed using a clinical MRI scanner OPTIMA 360 manufactured by GEMS. Due to the size of the tested objects, it was necessary to design additional receiving circuits allowing for the study of MCF-7 cell cultures placed in glass bioreactors. The examined sample's volume did not exceed 2.0 mL nor did the number of cells exceed 1 million. This work also included a modification of the sequence to allow for the analysis of T and T relaxation times. The analysis was performed using the MATLAB package (produced by MathWorks). The created application is based on medical MR images saved in the DICOM3.0 standard which ensures that the data analyzed are reliable and unchangeable in an unintentional manner that could affect the measurement results. The possibility of using 1.5 T MRI systems for cell culture research providing quantitative information from in vitro studies was realized. The scanning resolution for FOV = 5 cm and the matrix was achieved at a level of resolution of less than 0.1 mm/pixel. Receiving elements were built allowing for the acquisition of data for MRI image reconstruction confirmed by images of a phantom with a known structure and geometry. Magnetic resonance sequences were modified for the saturation recovery (SR) method, the purpose of which was to determine relaxation times. An application in MATLAB was developed that allows for the analysis of T and T relaxation times. The relaxation times of cell cultures were determined over a 6-week period. In the first week, the T time value was 1100 ± 40 ms, which decreased to 673 ± 59 ms by the sixth week. For T, the results were 171 ± 10 ms and 128 ± 12 ms, respectively.
这项工作的目的是使用并优化一台1.5特斯拉磁共振成像（MRI）系统，以获取体外培养的乳腺细胞小样本的三维（3D）图像。本研究的基础是设计MRI设备，以便能够对置于1.5毫升和2毫升玻璃管及/或外径小于20毫米的生物反应器中的MCF-7乳腺癌细胞培养物进行成像。此外，还描述了用于计算纵向和横向弛豫时间的软件的开发。成像测试使用了由GE医疗系统公司生产的临床MRI扫描仪OPTIMA 360进行。由于测试对象的尺寸，有必要设计额外的接收电路，以便对置于玻璃生物反应器中的MCF-7细胞培养物进行研究。所检查样本的体积不超过2.0毫升，细胞数量也不超过100万个。这项工作还包括对序列进行修改，以便能够分析T1和T2弛豫时间。分析使用MATLAB软件包（由MathWorks公司生产）进行。所创建的应用程序基于以DICOM3.0标准保存的医学MR图像，这确保了所分析的数据可靠且不会因可能影响测量结果的意外方式而改变。实现了使用1.5T MRI系统进行细胞培养研究并从体外研究中提供定量信息的可能性。当视野（FOV）=5厘米且矩阵时，扫描分辨率达到了小于0.1毫米/像素的水平。构建了接收元件，以便通过具有已知结构和几何形状的体模图像来确认采集用于MRI图像重建的数据。对磁共振序列进行了修改，采用饱和恢复（SR）方法，其目的是确定弛豫时间。开发了一个MATLAB应用程序，可用于分析T1和T2弛豫时间。在为期6周的时间内测定了细胞培养物的弛豫时间。在第一周，T1时间值为1100±40毫秒，到第六周降至673±59毫秒。对于T2，结果分别为171±10毫秒和128±12毫秒。
