Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Hum Pathol. 2012 Jun;43(6):808-17. doi: 10.1016/j.humpath.2011.07.018. Epub 2011 Nov 12.
We recently have demonstrated nuclear localization of E-cadherin in ovarian adult granulosa cell tumors (Histopathology 2011;58:423). The purpose of the present study is to investigate the diagnostic utility of E-cadherin nuclear staining for the differential diagnosis between ovarian adult granulosa cell tumor and its morphological mimics. Tissue samples taken from 81 ovarian tumors and 20 extraovarian tumors were immunohistochemically stained using monoclonal anti-E-cadherin antibody recognizing cytoplasmic domain (clone 36 supplied by BD Biosciences, San Jose, CA). The ovarian tumors consisted of 30 adult granulosa cell tumors, 3 Sertoli-stromal cell tumors, 14 fibrothecomas, 5 carcinoid tumors, 1 large cell neuroendocrine carcinoma, 18 endometrioid adenocarcinomas, and 10 poorly differentiated serous adenocarcinomas. Extraovarian tumors consisted of 16 uterine endometrial stromal neoplasms and 4 pulmonary small cell carcinomas. Only tumor cells with nuclear staining were considered positive in this study. Ninety percent of adult granulosa cell tumors, 67% of Sertoli-stromal cell tumors, 64% of fibrothecomas, 75% of endometrial stromal neoplasms, 75% of small cell carcinomas, and the one large cell neuroendocrine carcinoma showed E-cadherin nuclear expression, whereas all the ovarian carcinoid tumors, endometrioid adenocarcinomas, and poorly differentiated serous adenocarcinomas were negative. E-cadherin nuclear staining is useful in distinguishing between adult granulosa cell tumors and ovarian adenocarcinomas or carcinoid tumors. However, it is of limited use for distinguishing between adult granulosa cell tumors and endometrial stromal neoplasms or small cell carcinomas. E-cadherin should be included in the immunohistochemical panel for an accurate diagnosis of ovarian adult granulosa cell tumors.
我们最近证明了 E-钙黏蛋白在卵巢成人颗粒细胞瘤中的核定位（Histopathology 2011;58:423）。本研究旨在探讨 E-钙黏蛋白核染色在卵巢成人颗粒细胞瘤及其形态学模拟物鉴别诊断中的应用价值。对 81 例卵巢肿瘤和 20 例卵巢外肿瘤组织标本进行了免疫组织化学染色，使用单克隆抗 E-钙黏蛋白抗体（BD Biosciences，San Jose，CA 提供的克隆 36）识别细胞质结构域。卵巢肿瘤包括 30 例成人颗粒细胞瘤、3 例 Sertoli-间质细胞瘤、14 例纤维卵泡膜细胞瘤、5 例类癌瘤、1 例大细胞神经内分泌癌、18 例子宫内膜样腺癌和 10 例低分化浆液性腺癌。卵巢外肿瘤包括 16 例子宫子宫内膜间质肿瘤和 4 例肺小细胞癌。在这项研究中，只有具有核染色的肿瘤细胞被认为是阳性的。90%的成人颗粒细胞瘤、67%的 Sertoli-间质细胞瘤、64%的纤维卵泡膜细胞瘤、75%的子宫内膜间质肿瘤、75%的小细胞癌和 1 例大细胞神经内分泌癌显示 E-钙黏蛋白核表达，而所有的卵巢类癌瘤、子宫内膜样腺癌和低分化浆液性腺癌均为阴性。E-钙黏蛋白核染色有助于区分成人颗粒细胞瘤和卵巢腺癌或类癌瘤。然而，对于区分成人颗粒细胞瘤与子宫内膜间质肿瘤或小细胞癌，其应用有限。E-钙黏蛋白应包含在卵巢成人颗粒细胞瘤的免疫组化检测中，以进行准确诊断。
