Ghost Laboratory, T Cell Memory and Tolerance Section, Laboratory of Cellular and Molecular Immunology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
J Immunol. 2011 Dec 15;187(12):6235-42. doi: 10.4049/jimmunol.1101540. Epub 2011 Nov 11.
In this study, we describe a new population of NK cells that reside in the normal, uninflamed peritoneal cavity. Phenotypically, they share some similarities with the small population of CD49b(-), CD27(+) immature splenic NK cells, as well as liver NK cells, but they differ in their expression of CD62L, TRAIL, and EOMES. Functionally, the peritoneal NK cells resemble the immature splenic NK cells in their production of IFN-γ, GM-CSF, and TNF-α and in the killing of YAC-1 target cells. We also found that the peritoneum induces different behavior in mature and immature splenic NK cells. When transferred i.v. into RAGγc knockout mice, both populations undergo homeostatic proliferation in the spleen, but only the immature splenic NK cells are able to reach the peritoneum. When transferred directly into the peritoneum, the mature NK cells survive but do not divide, whereas the immature NK cells proliferate profusely. These data suggest that the peritoneum is not only home to a new subset of tissue-resident NK cells, but that it differentially regulates the migration and homeostatic proliferation of immature versus mature NK cells.
在这项研究中，我们描述了一种存在于正常、非炎症性腹腔内的新型 NK 细胞群体。表型上，它们与少量 CD49b(-)、CD27(+)未成熟脾 NK 细胞以及肝 NK 细胞具有一些相似之处，但在 CD62L、TRAIL 和 EOMES 的表达上有所不同。功能上，腹膜 NK 细胞在产生 IFN-γ、GM-CSF 和 TNF-α以及杀伤 YAC-1 靶细胞方面与未成熟脾 NK 细胞相似。我们还发现，腹膜诱导成熟和未成熟脾 NK 细胞表现出不同的行为。当静脉转移到 RAGγc 敲除小鼠中时，这两种细胞群都在脾脏中经历了稳态增殖，但只有未成熟的脾 NK 细胞能够到达腹膜。当直接转移到腹膜中时，成熟 NK 细胞存活但不分裂，而未成熟 NK 细胞则大量增殖。这些数据表明，腹膜不仅是组织驻留 NK 细胞的一个新亚群的所在地，而且还能差异化调节未成熟与成熟 NK 细胞的迁移和稳态增殖。
